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Mango leaf extract, its preparation method and use

A mango leaf and extract technology, applied in the field of mango leaf extract and its preparation, can solve the problems of inability to prevent and treat diabetes and lower blood fat, and difficulty in obtaining mango leaf extract

Active Publication Date: 2016-01-27
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Refining methods include the use of macroporous adsorption resin or silica gel chromatography. Silica gel chromatography uses a large amount of organic solvents and is not suitable for large-scale production. In the past, macroporous adsorption resin purification methods used high-concentration ethanol for elution, but it was difficult to obtain Mango leaf extract with a mangiferin content of more than 50%, more importantly, the eluted mango leaf extract cannot achieve the effects of preventing diabetes and lowering lipids

Method used

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  • Mango leaf extract, its preparation method and use
  • Mango leaf extract, its preparation method and use
  • Mango leaf extract, its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1: the preparation of mango leaf extract sample 1

[0065] refer to figure 1 Sample 1 was prepared by the procedure shown. Specific steps are as follows:

[0066] Accurately weigh 100g of mango leaf powder, add 8 times the amount of 70% ethanol solvent, soak for 1 hour, boil and reflux for extraction for 2 hours, filter while hot, continue to add 8 times the amount of 70% ethanol solvent to the filter residue, continue to reflux for 2 hours, and combine The 2 filtrates were left to stand at room temperature, and the volume of the extract was accurately measured to a total of 1.39L, and 1mL was taken for HPLC detection to determine the content of mangiferin in the mango leaf extract. The rest of the solution was recovered with ethanol, concentrated to the maximum, and dried under vacuum at 60° C. to obtain sample 1 of mango leaf extract.

Embodiment 2

[0067] Embodiment 2: the preparation of mango leaf extract sample 2

[0068] refer to figure 2 Sample 2 was prepared by the procedure shown. Specific steps are as follows:

[0069]Accurately weigh 100g of mango leaf powder, add 8 times the amount of 70% ethanol solvent, soak for 1 hour, boil and reflux for extraction for 2 hours, filter while hot, continue to add 8 times the amount of 70% ethanol solvent to the filter residue, continue to reflux for 2 hours, and combine The 2 filtrates were allowed to stand at room temperature, and the total volume of the extract was accurately measured to be 1.3 L, concentrated to 200 mL according to the ratio of crude drug (g): solution volume (mL) = 1:2, and an equal volume of ethyl acetate was added to mix Evenly, extract for 30 minutes, release the ethyl acetate layer after extraction and separation, continue to add an equal volume of ethyl acetate to the water layer and mix, extract for 30 minutes, release the ethyl acetate layer af...

Embodiment 3

[0070] Embodiment 3: the preparation of mango leaf extract sample 3

[0071] refer to image 3 Sample 3 was prepared according to the procedure shown. Specific steps are as follows:

[0072] Accurately weigh 100g of mango leaf powder, add 8 times the amount of 70% ethanol solvent, soak for 1 hour, boil and reflux for extraction for 2 hours, filter while hot, continue to add 8 times the amount of 70% ethanol solvent to the filter residue, continue to reflux for 2 hours, and combine 2 filtrates, let stand at room temperature, accurately measure the total volume of the extract 1.7L, concentrate to 200mL according to the ratio of crude drug (g): solution volume (mL) = 1:2, centrifuge at 3000 rpm for 15min and discard precipitation. Accurately measure 200mL of pretreated D101 macroporous adsorption resin, and put it on the column by wet method. Take the processed concentrated solution and load it with a flow rate of 40min / BV, pre-elute with 3BV of water, 5 times the amount of...

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Abstract

The invention belongs to the field of traditional Chinese medicine and medicinal chemistry, relates to a mango leaf extractive as well as a preparation method and application thereof and particularly relates to a preparation method of the mango leaf extractive. The method comprises the following steps of: (1) extracting mango leaves by using an organic solvent to obtain an extracting solution; (2) concentrating the extracting solution in the step (1) and centrifuging the concentrated extracting solution to obtain a supernatant liquid; (3) eluting macroporous resin on the supernatant liquid in the step (2) to obtain an eluent; and (4) concentrating the eluent in the step (3) and drying the concentrated eluent to obtain a dry substance, namely the extractive. The invention also relates to the mango leaf extractive obtained by using the preparation method and an application of the mango leaf extractive to the preparation of medicaments for preventing and treating diabetes mellitus or hyperlipidemia.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine and medicinal chemistry, and relates to a mango leaf extract, its preparation method and application. Background technique [0002] Mango leaves are the leaves of Mango Mangiferaindica L. of Anacardiaceae. According to the "Dictionary of Chinese Medicine" records: mango folic acid, sweet, cool in nature. Promoting qi and dispersing stagnation, removing diarrhoea; treating abdominal pain and flatulence caused by heat stagnation; and washing rotten sores. Its main component, mangiferin, is the main active ingredient in the treatment of respiratory diseases, and has been found in other plants. Modern pharmacological studies have shown that mango leaf extract has the effects of relieving asthma, relieving cough and expectorant, immune, anti-inflammatory and analgesic, anti-oxidation, anti-diabetic, antibacterial and antiviral. At present, there are mango cough tablets, mango cough capsul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/22A61P3/10A61P3/06A61K127/00
Inventor 张袆王涛刘二伟郭玲玲胡利民高秀梅
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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