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Preparation method of seed liquid and preparation method of ethanol

A seed and seed tank technology, applied in the field of seed liquid preparation, can solve the problems of unsatisfactory antibacterial effect, increased production cost, and unsatisfactory control effect, so as to reduce the acid rise difference, prevent the infection of miscellaneous bacteria, optimize the quality effect

Active Publication Date: 2015-05-27
COFCO GROUP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main control measure is to add bacteriostatic agents regularly during the cultivation process (that is, after mixing the medium with yeast, add bactericides to the mixed mixture every 2 hours) to control miscellaneous bacteria reproduction, but the control effect is not ideal
The reason is that, from the perspective of long-term production operation, even if the addition of antibacterial agents in the initial cultivation stage can inhibit the growth of miscellaneous bacteria to a certain extent, the drug resistance of miscellaneous bacteria will gradually increase in the later stage of cultivation, so it is necessary to further improve the antimicrobial agent. This will not only increase the production cost, but also the antibacterial effect is still not ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0009] According to the present invention, the preparation method of the seed liquid includes expanding the culture of the yeast, wherein, the method of expanding the culture of the yeast includes: mixing the culture medium with the yeast in a seed tank in a continuous feeding mode , cultivation, and the seed liquid obtained after the expanded cultivation is continuously drawn from the seed tank; a bactericide is added during the expanded cultivation process, and the addition method and amount of the bactericide make the number of miscellaneous bacteria in the entire expanded cultivation process Observe no more than 2 per field of view under a microscope at 1000 times.

[0010] According to the present invention, the microscope can be various microscopes conventionally used in this field, such as optical microscope. The counting method can be a conventional counting method in the art, such as using a hemocytometer to count.

[0011] According to the present invention, prefera...

Embodiment 1

[0033] This example is used to illustrate the preparation method of the seed liquid provided by the present invention.

[0034] The medium is continuously added to the seed tank at a flow rate of 45 cubic meters per hour and mixed with the yeast activation solution (relative to each kilogram of yeast activation solution, the amount of medium added is 3 cubic meters), and the medium is heated at 25 ° C. , air flow is 0.5 cubic meters of air: cultivate under cubic meter of seed liquid per minute, and the mixture obtained after expanding the cultivation is continuously drawn from the described seed tank; Under the condition of 3.6, add the first batch of penicillin bactericide in the seed tank; when the amount of the culture medium that feeds on reaches 40% of the seed tank volume, add the second batch of penicillin bactericide in the seed tank; When the amount of base reached the liquid level of the discharge of the seed tank, the third batch of penicillin bactericide was added ...

Embodiment 2

[0039] This example is used to illustrate the preparation method of the seed liquid provided by the present invention.

[0040] The culture medium is continuously added to the seed tank at a flow rate of 60 cubic meters per hour and mixed with the yeast activation solution (relative to each kilogram of yeast activation solution, the amount of the medium added is 3 cubic meters), and at 25 ° C , air flow is 0.6 cubic meters of air: under the cubic meter of seed liquid · minute, cultivate, and the mixture obtained after expanding the cultivation is drawn continuously from the described seed tank; Under the condition of 3.8, add the first batch of penicillin bactericide in the seed tank; when the amount of the culture medium that fed-batch reaches 40% of the seed tank volume, add the second batch of penicillin bactericide in the seed tank; When the amount of base reached the liquid level of the discharge of the seed tank, the third batch of penicillin bactericide was added in the...

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Abstract

The invention provides a preparation method of a seed liquid and a preparation method of ethanol. The preparation method of the seed liquid comprises the step of amplifying culture of yeast, wherein the method for amplifying culture of yeast comprises the steps of mixing a culture medium with the yeast in a seeding tank in a continuous feeding manner for culture, and leading the seed liquid obtained after the amplifying culture out of the seeding tank continuously; and adding a bactericide in the amplifying culture process, wherein the adding manner and adding quantity of the bactericide enable the contaminating microorganism quantity to be smaller than 2 per visual field when the contaminating microorganisms are observed under a microscope of 1,000 times in the whole amplifying culture process. The preparation method of the seed liquid can ensure that a volatile acid of the prepared seed liquid is controlled within 0.05, thereby optimizing the quality of the seed liquid; and further, the acidity rising diffidence can be reduced in a follow-up alcoholic fermentation preparation process, and the ethanol yield can be improved.

Description

technical field [0001] The invention relates to a method for preparing seed liquid and a method for preparing ethanol. Background technique [0002] In the alcohol fermentation production industry, the control of miscellaneous bacteria is particularly important during the cultivation of yeast. At present, the main control measure is to add bacteriostatic agents regularly during the cultivation process (i.e., after mixing the medium with yeast, add bactericide to the mixed mixture every 2 hours) to control miscellaneous bacteria reproduction, but the control effect is not ideal. The reason is that, from the perspective of long-term production operation, even if the addition of antibacterial agents in the initial cultivation stage can inhibit the growth of miscellaneous bacteria to a certain extent, however, the drug resistance of miscellaneous bacteria will gradually increase in the later stage of cultivation, so it is necessary to further improve the antimicrobial agent. T...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/18C12P7/06C12R1/865
CPCY02E50/10
Inventor 柳树海刘玉华范新龙杜金宝梁春丽张武春林海龙
Owner COFCO GROUP