Reagent device and method for detecting varicella-zoster-virus antibody

Abstract

The invention provides a reagent device and a method used for detecting varicella-zoster-virus antibodies. The reagent device has a long strip shape, and has a base body comprising 8 hole positions and a handle positioned on one end of the base body. From the end proximal to the handle, the 8 hole positions are sequentially a sample hole, an auxiliary agent hole, an enzyme conjugate hole, a substrate hole, a termination liquid hole, a dilution liquid hole, a reaction hole, and a dilution hole. According to the invention, based on a principle of enzyme-linked immunoassay, varicella-zoster-virus antibody is detected by using the reagent device. The method is an independent single-person analysis and detection method. The device can be used in cooperation with a corresponding specific analysis instrument. During a detection process, detection reagents or samples are injected by using a full-automatic precise dosing device. The device and the method have the advantages of automatic operation, precise dosing, high detection result accuracy, high detection result precision, and wide application prospect.

Description

Technical field [0001] The application of the present invention relates to a reagent device and method for detecting varicella-zoster virus antibodies, and belongs to the technical field of clinical immunological detection. Background technique [0002] Varicella-Zoster virus (Varicella-Zoster virus, VZV) has a spherical shape with a diameter of 150nm~200nm. The complete virus consists of a core, a nucleocapsid and an envelope. The core of the virus is double-stranded DNA with a small amount of enzyme protein. The core-shell is composed of 162 hollow tubular shell particles to form a octahedron. The outermost layer is the envelope, which is composed of fat and glycoprotein, and the antigenic structure exists in the glycoprotein. [0003] Chickenpox is caused when children are first infected with varicella-zoster virus, and the virus in the latent body recurs after some stimulation and causes herpes zoster, which is more common in adults and the elderly. Varicella occurs in child...

AI Technical Summary

Problems solved by technology

[0008] (1) Non-specific recognition cannot be distinguished according to the size of the molecular weight when analyzing the results;

[0009] (2) The operation is relatively complicated and requires expensive fluorescence microscopes, which are difficult to popularize in many primary hospitals and are not suitable for laboratories with a large number of specimens;

[0010] (3) The background in the fluorescence measurement is relatively high, and it is difficult to use the fluorescence immunoassay technique for quantitative determination;

[0011] (4) Experienced professionals are required to determine the results, and the objectivity of the analysis results is insufficient;

[0012] (5) Only qualitative testing can be performed, not quantitative testing

[0015] (1) Use 12×8 type, 6×8 type, 8×12 type or whole plate type 96-well special microwell plate as antigen coating equipment and reaction Containers can only be divided into 12 batches, 6 batches, 8 batches or the whole board for one-time use, and independent and single-person testing cannot be carried out;

[0016] (2) There are many types of reagents used in the determination, and each detection reagent must be filled with a reagent bottle, and each time a reagent is used, it is necessary to replace the suction nozzle to add to the microwells of the microwell plat...

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