Synchronous preparation method of effective ingredient of licorice

A technology of licorice root and total flavonoids of licorice root is applied in the field of extraction, separation and purification of natural products, and can solve the problems that the extraction and separation steps are unsuitable for industrialized production, are not economical, environmentally friendly, etc., so as to improve comprehensive utilization rate, facilitate industrialized production, and reduce costs. Effect

Active Publication Date: 2013-07-17
ELION RESOURCE GROUP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the existing extraction and separation technology is single or intermittent, often simply extracting glycyrrhizin saponin, licorice residue is discarded, or extracting licorice flavonoids from the residue after extraction, and then extracting licorice polysaccharide
The Chinese patent "A Method for Extracting and Separating Glycyrrhizic Acid, Glycyrrhizic Flavonoids and Glycyrrhizic Polysaccharides" (CN1803789) discloses a method for extracting glycyrrhizic acid, flavonoids and polysaccharides from licorice, but a large amount of organic solvents are used in the process, which is not economical and Environmental friendly
The Chinese patent "Systematic separation and extraction of licorice flavonoids, glycyrrhizic acid, and licorice polysaccharides from licorice" (CN1359905A) discloses a method for systematically extracting glycyrrhizic acid, licorice flavonoids, and licorice polysaccharides, but too many extraction and separation steps are not suitable for industrialization Production

Method used

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Examples

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Comparison scheme
Effect test

preparation example Construction

[0024] The synchronous preparation method of active ingredient of Radix Glycyrrhizae of the present invention, preferred specific steps are:

[0025] 1) One-time extraction: crush the raw licorice to 10-120 mesh, add 60%-95% ethanol aqueous solution with a concentration 6-10 times the weight of licorice, soak for 2-3 hours, and keep warm at a slight boiling state (80-100°C) 1~2 h, filter, collect the primary extract and filter residue; secondary extraction: filter residue with 20%~60% ethanol aqueous solution at a concentration of 6~10 times the weight of licorice, and keep warm for 1~2 h, extracting 1 to 5 times, the extracts are collected and combined to obtain a secondary extract; the primary extract and the secondary extract are combined;

[0026] 2) The extract from step 1) is passed through a 300-400 mesh filter bag while it is hot to obtain a clarified filtrate. The filtrate sequentially passes through two macroporous resin high pressure columns of different polarities...

Embodiment 1

[0045] 1) One-time extraction: crush 100 g of licorice as raw material to 50 mesh, add 8 times the weight of licorice in 60% ethanol aqueous solution and soak for 2-3 hours, keep warm for 1-2 hours in a slightly boiling state, filter and collect the primary extract and Filter residue; secondary extraction: filter residue with 6 times the concentration of 30% ethanol aqueous solution in a slightly boiling state for 1~2 h, and then extract twice (specific process: filter residue with 6 times the concentration of 30% ethanol aqueous solution Insulate for 1~2 h in a slightly boiling state, filter, collect the extract a and the secondary filter residue, and then use 6 times the amount of 30% ethanol aqueous solution to keep the temperature in a slightly boiling state for 1~2 h, filter, Collect the extract b, combine the extracts a and b to obtain the secondary extract), collect the secondary extract; combine the primary extract and the secondary extract.

[0046] 2) Step 1) The obt...

Embodiment 2

[0049] 1) One-time extraction: Grind 1 kg of licorice as a raw material to 50 mesh, add 6 times the weight of licorice in 95% ethanol aqueous solution and soak for 2-3 hours, keep warm for 1-2 hours in a slightly boiling state, filter and collect the extract and filter residue once ; The filter residue was incubated with 10 times the amount of 20% ethanol aqueous solution in a slightly boiling state for 1-2 h, and extracted 3 times accordingly, and the secondary extract was collected; the primary extract and the secondary extract were combined.

[0050] 2) Step 1) The obtained extract is passed through a 300-400 mesh filter bag while it is hot to obtain a clarified filtrate. The filtrate first passed through the high-pressure column equipped with PIPO-00 macroporous resin (purchased from Guangzhou Paipai Biotechnology Co., Ltd.), and then passed through the HPD-500 macroporous resin high-pressure column (the weight of the macroporous resin in the two resin columns equal), coll...

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Abstract

The invention discloses a synchronous preparation method of an effective ingredient of licorice, wherein the method comprises the following steps that: 1) licorice is soaked with an aqueous ethanol solution and then is subjected to immersing boiling extraction; 2) the extract solution is filtered, and the effluent is collected through two sections of macroporous resin columns connected in series and having different polarities, wherein the macroporous resin columns connected in series are that a nonpolar macroporous resin column is connected in series with a moderately polar or polar macroporous resin column, or a polar macroporous resin column is connected in series with a nonpolar macroporous resin column; 3) the resin column is rinsed with water to remove impurity, the nonpolar macroporous resin column is eluted with a 70-90% aqueous ethanol solution, and the moderately polar or polar macroporous resin column is eluted twice sequentially through a 30-40% aqueous ethanol solution and a 50-70% aqueous ethanol solution; and 4) the effluent and various eluents are respectively dried to obtain licorice total polysaccharides, licorice isopentenyl flavonoids, licorice total flavonoids excluding isopentenyl, and licorice total saponins. By the invention, substances with different polarities in licorice are separated, separation efficiency is improved, and waste of solvent is avoided.

Description

technical field [0001] The invention belongs to the field of extraction, separation and purification of natural products, in particular to a method for synchronous preparation of effective components of licorice, in particular to synchronous extraction and separation of prenyl flavones, total flavones and total saponins other than prenyl flavones from licorice and total polysaccharides. Background technique [0002] Traditional Chinese medicine theory believes that licorice is sweet in taste and flat in nature. It has the effects of nourishing the spleen and nourishing qi, clearing away heat and detoxification, eliminating phlegm and relieving cough, relieving spasms and relieving pain, and harmonizing various medicines. Pharmacological experiments have confirmed that licorice has liver protection, anti-inflammatory, antibacterial, antiviral, immune regulation, hypoglycemic and anti-platelet aggregation effects. Its main active ingredients are flavonoids, saponins and polys...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07D311/40C08B37/00A61K36/484A61P31/04A61P31/12A61P35/00A61P39/06A61P39/02A61P11/06A61P11/14A61P29/00A61P37/08A61P1/16A61P3/06
Inventor 朱贺年李卫民
Owner ELION RESOURCE GROUP
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