Clenbuterol, ractopamine and salbutamol three-in-one euzyme linked immunosorbent assay, special kit and kit detection method
An enzyme-linked immunoassay and ractopamine technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of radioisotope radiation and pollution, difficult waste disposal, lack of detection sensitivity, etc., and achieve simplified operation steps and reaction time , low cost, improved precision and accuracy
Active Publication Date: 2013-07-31
GUANGZHOU RUISEN BIOTECH
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Problems solved by technology
Radioimmunoassay technology has the advantages of strong specificity, high sensitivity, accuracy, speed, easy operation, and easy standardization, etc., but the instruments used are expensive, and the radioisotopes used are irradiated and polluted, and the waste is not easy to handle, which limits this method. Wide application of the method; gold standard immunoassay technology can screen a large number of samples, but its false positive rate is high, the detection sensitivity is insufficient, the stability is insufficient, and it cannot provide accurate results
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[0039] The enzyme-linked immunoassay method for the one-time detection of clenbuterol, ractopamine and salbutamol of the present invention is to pre-coat monoclonal antibodies on the enzyme plate, and the clenbuterol, salbutamol and ractopamine in the sample to be tested, After the enzyme-labeled antigen competes with the coated monoclonal antibody, the color is developed with a chromogenic agent. The content of clenbuterol, ractopamine, and salbutamol in the sample to be tested is inversely proportional to the percent absorbance value of the sample to be tested. Compared with the standard curve, the content of clenbuterol, albuterol, and ractopamine in the sample to be detected can be obtained. The logarithmic values of clenbuterol, ractopamine, and albuterol standard substance concentrations are plotted on the abscissa, and the pre-coated monoclonal antibody can cross-react simultaneously to clenbuterol, ractopamine, and albuterol. The absorbance value was calculated accor...
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Abstract
The invention discloses a clenbuterol, ractopamine and salbutamol disposable method for euzyme linked immunosorbent assay detection, which is characterized in that a monoclonal antibody is precoated on an enzyme label plate, clenbuterol, ractopamine and salbutamol in a sample to be detected and enzyme-labelled antigen compete for binding with the coated monoclonal antibody, a developer is used for developing, the content of clenbuterol, ractopamine and salbutamol in the sample to be detected is in inverse proportion with percent absorbency of the sample to be detected, compared with a standard curve, the content of clenbuterol, ractopamine and salbutamol in the sample to be detected can be obtained, and a special detection kit used for the above mentioned detection method comprises the enzyme label plate, a standard substance solution, a concentrated enzyme marker, an enzyme marker diluents, the developer, a concentrated washing lotion and a stopping solution. The invention uses a direct competition enzyme linked immunosorbent assay technology, three kinds of clenbuterol hydrochloride which are clenobuterol hydrochloride, ractopamine and salbutamol are performed with disposable analysis detection, the usage is simple, the specificity is high, and the detection scope is 0.1ng / mL-2mumg / mL.
Description
technical field [0001] The invention relates to the technical field of beta stimulant detection, in particular to an enzyme-linked immunoassay for one-time detection of clenbuterol, ractopamine and salbutamol, a special detection kit and a detection method thereof. Background technique [0002] With the continuous improvement of people's living standards, people begin to pay more and more attention to their health. In daily life, people often cook meat to enhance their immunity and supplement nutrition. When eating food, people often worry about a question-"Does meat food contain beta stimulants (clenbuterol)?" Beta stimulants are pharmaceutical additives that can promote lean meat growth, such as clenbuterol hydrochloride That is, clenbuterol hydrochloride, ractopamine, salbutamol, etc. It can increase the growth rate of pigs, increase the percentage of lean meat, and can make the color of pig hair ruddy and bright, and it looks good; after slaughter, the color of the meat...
Claims
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Inventor 罗奕铭
Owner GUANGZHOU RUISEN BIOTECH