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Culture medium for producing amylase by using deep marine geobacillus sp (Geobacillus sp.)

A thermophilic bacteria, culture medium technology, applied in the biological field, can solve the problems of difficult enzymatic research and industrial production, low amylase yield, etc.

Active Publication Date: 2015-03-11
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the amylase yield of the thermophilic bacterium Geobacillus sp.4j in the original medium is very low, which has become a bottleneck in various researches, especially in the field of enzyme production in its large-scale fermentation culture
If the yield of amylase cannot be increased, it will be difficult to carry out corresponding enzymatic research and exploration of industrial production

Method used

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  • Culture medium for producing amylase by using deep marine geobacillus sp (Geobacillus sp.)
  • Culture medium for producing amylase by using deep marine geobacillus sp (Geobacillus sp.)
  • Culture medium for producing amylase by using deep marine geobacillus sp (Geobacillus sp.)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Embodiment 1, culture medium 1

[0068] 1) Gelatinization of soluble starch

[0069] Weigh 10g of soluble starch, make a suspension with 20ml of deionized water, then slowly pour it into freshly boiled deionized water and keep stirring to make a translucent uniform viscous liquid, and cool it down.

[0070] 2) Culture medium preparation

[0071] Weigh 4g of yeast powder, 3g of sodium nitrate, 20g of sodium chloride, 1.67g of anhydrous magnesium sulfate, 8.92g of magnesium chloride, 0.33g of potassium chloride, 0.1g of calcium chloride, 0.183g of dipotassium hydrogen phosphate, and 0.01 g of ferrous ammonium sulfate g, pour an appropriate amount of deionized water to fully dissolve the above components, then pour into the cooled starch gelatinization solution prepared in step 2), add 10ml of trace element solution, 50 μL of nickel chloride solution (10g / L), and then use deionized The volume of water was adjusted to 1000ml, and the pH was adjusted to 6.

[0072] Wherei...

Embodiment 2

[0079] Embodiment 2, culture medium 2

[0080] 1) Gelatinization of soluble starch

[0081] Make a suspension of 10g of soluble starch with 20ml of deionized water, then slowly pour it into freshly boiled deionized water and keep stirring to make a translucent uniform viscous liquid, and cool it down.

[0082] 2) Culture medium preparation

[0083] Weigh 8g of yeast powder, 1g of sodium nitrate, 20g of sodium chloride, 1.67g of anhydrous magnesium sulfate, 15g of magnesium chloride, 0.33g of potassium chloride, 0.15g of calcium chloride, 0.183g of dipotassium hydrogen phosphate, and 0.01g of ferrous ammonium sulfate , pour an appropriate amount of deionized water to fully dissolve the above components, then pour into the cooled starch gelatinization solution prepared in step 1), add trace element solution I10ml, nickel chloride solution (10g / L) 50μL, and then use deionized water The volume was adjusted to 1000ml, and the pH was adjusted to 6.

[0084] 3) Fermentation and cult...

Embodiment 3

[0088] Embodiment 3, culture medium 3

[0089] 1) Gelatinization of soluble starch

[0090] Weigh 9g of soluble starch and make a suspension with 20ml of deionized water, then slowly pour it into freshly boiled deionized water and stir continuously to make a translucent uniform viscous liquid, and cool it down.

[0091] 2) Culture medium preparation

[0092] Weigh 8g of yeast powder, 7.5g of sodium nitrate, 20g of sodium chloride, 1.67g of anhydrous magnesium sulfate, 6g of magnesium chloride, 0.33g of potassium chloride, 0.6g of calcium chloride, 0.2g of dipotassium hydrogen phosphate, and 0.01 g of ferrous ammonium sulfate. g, pour an appropriate amount of deionized water to fully dissolve the above ingredients, then pour into the cooled starch gelatinization solution prepared in step 1), add 12ml of trace element solution, 50 μL of nickel chloride solution (10g / L), and then use deionized The volume of water was adjusted to 1000ml, and the pH was adjusted to 6.

[0093] 3...

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Abstract

The invention relates to a culture medium for producing amylase by using deep marine geobacillus sp (Geobacillus sp.). The culture medium is suitable for the growth of the deep marine geobacillus sp (Geobacillus sp.) and can greatly increase the output of the amylases.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically, the invention relates to a culture medium for deep-sea marine thermophilic bacteria Geobacillus sp. (better Geobacillus sp.4j) to produce amylase and a preparation method thereof. Background technique [0002] Thermophilic marine microorganisms are a type of thermophilic microbial resources derived from special environments such as geothermal oil-bearing geological formations, deep-sea volcanic mud, and deep-sea hydrothermal vents. Thermophilic microorganisms have attracted much attention because of their unique adaptability to high temperatures, and generally refer to a class of microorganisms whose optimum growth temperature is greater than 45°C. As a thermophilic microorganism, one of its main applications is the development and application of thermophilic enzymes. Thermophilic enzymes have attracted much attention because of their high thermostability. The most famous of t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/26C12R1/01
Inventor 周祥山蒋涛蔡孟浩张元兴
Owner EAST CHINA UNIV OF SCI & TECH