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Rapid propagation and transplantation method of blumea balsamifera

A kind of Ainaxiang, fast technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effect of ensuring survival rate and low cost

Inactive Publication Date: 2013-08-21
GUIZHOU YIHE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are a few reports on the technology of tissue culture and rapid propagation of Ainaia plants

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] ① Anti-browning and disinfection treatment of explants: take young leaves that have germinated for 5-10 days from March to April, rinse the leaves, and use distilled aqueous solution of 3000 mg / L vitamin C + 2000 mg / L gentamicin Soak for 60 minutes, then soak in 2000mg / L potassium permanganate distilled aqueous solution for 30 minutes, rinse with distilled water for 3-5 times, and trim the young leaves on the ultra-clean workbench to a petiole length of 0.2-0.5cm and a leaf area of ​​0.5-1.0cm 2 The explants were disinfected with 1000 mg / L mercuric chloride aqueous solution for 10 minutes, and rinsed with clean water 4-5 times.

[0019] ②The same basic medium is used in each stage: 1 / 2MS+12g / L agar+30g / L sucrose, pH 6.0;

[0020] The culture conditions at each stage are: temperature 24-26°C, light 1500-2000Lux, light time 12-14hr / d.

[0021] ③Initial induction culture of explants: insert the petiole base of the sterilized leaf explants into the basic medium (1 / 2MS+12g / ...

Embodiment 2

[0028] ① Anti-browning and disinfection treatment of explants: Take young stem segments with 5-6 young leaves in March-April, and put

[0029]After the stems are washed clean, soak them in a distilled aqueous solution of 3000 mg / L vitamin C + 2000 mg / L gentamycin for 60 minutes, then soak them in a distilled aqueous solution of 2000 mg / L potassium permanganate for 30 minutes, rinse them with distilled water for 3-5 times, and The stem segment was trimmed to a 0.5-1.0 cm long stem segment explant with 1 axillary bud on an ultra-clean workbench, then routinely disinfected with 1000 mg / L mercuric chloride aqueous solution for 10 minutes, and rinsed with clean water 4-5 times.

[0030] ② The same basic medium is used at each stage: 1 / 2MS+12g / L agar+30g / L sucrose, pH 5.8;

[0031] The culture conditions at each stage are: temperature 24-26°C, light 1500-2000Lux, light time 12-14hr / d.

[0032] ③Initial induction culture of explants: implant the sterilized stem explants into the bas...

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PUM

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Abstract

The invention discloses a rapid propagation and transplantation method of blumea balsamifera. The method comprises the technical links of explant browning resistance, disinfection, primary induction, mulitiplication, root induction, bottle seedling transplantation and the like, specifically comprising the following steps of: inducing multiple shoots by taking tender leaves with petioles or tender stems with axillary buds of blumea balsamifera as explants, and taking integrated leaves with stems of the multiple shoots as mulitiplication materials or direct root induction materials. The invention provides a whole set of rapid propagation and transplantation method of the blumea balsamifera. After the method is used, the browning rate and the pollution rate of the explant can be obviously reduced, the primary induction and inoculation survival rate of the explant is more than 90%, the mulitiplication period is 35 days, integrated leaves with the stems of the multiple shoots can be directly used for the root induction, and the rooting percentage reaches 100%. The bottle seedling transplantation method is simple and easy, the survival rate is more than 90%, and the technical guarantee can be provided for the shortage of the large-scale plantation of seedlings of the blumea balsamifera.

Description

technical field [0001] The invention relates to a rapid propagation and transplanting method of Ainaxiang. Background technique [0002] Asteraceae Compositae B1umea DC. Plants all over the world. About 80 species, distributed in tropical and subtropical Asia, Africa and Oceania; 30 species in my country, distributed in the provinces and regions south of the Yangtze River Basin; 14 species in Guizhou, and 8 plants of the genus Ainarum in Luodian species, of which Ainaxiang Bl. balsamifera (L.) DC, Qiantou Ainaxiang Bl. lanceolarla Roxb, soft hair Ainaxiang Bl. mollis (D.Don)Merr., See Frost Yellow Bl. lacera (Burm.f.) DC., six ear bells Bl. Laciniata (Roxb.) DC have good medicinal value. Ainaxiang is a perennial herb. The whole herb is used as medicine. It is mainly used to treat colds, rheumatoid arthritis, postpartum wind pain, and dysmenorrhea. The artificial cultivation of Ainaxiang has a long history. As early as around 1938, people in Luodian and Wangmo co...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G1/00
Inventor 谢丙质王济红欧国腾刘燕
Owner GUIZHOU YIHE BIOTECH
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