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Magnetic flocculant and application method thereof

A magnetic flocculant and application method technology, applied in the field of microbial fermentation, to achieve the effect of fast flocculation recovery speed and small impact

Inactive Publication Date: 2013-08-21
CHINA NAT OFFSHORE OIL CORP +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are rare reports on the use of magnetic flocculation technology in improving the recovery rate of fermentation broth cells, so this technology has great application potential in this field.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A magnetic flocculant, which is composed of flocculant and magnetic seed, the weight ratio of flocculant and magnetic seed is 1:1; among them, the flocculant is a high-efficiency flocculant, preferably polyaluminum chloride and poly A mixture of ferric sulfate, and the weight ratio of polyaluminum chloride and polyferric sulfate is 1:1; the magnetic species is Fe with a particle size of 100nm to 100um 3 o 4 magnetic powder.

[0016] Add the above-mentioned magnetic flocculant into the fermentation liquid rich in petroleum hydrocarbon degrading bacteria Ornithinimicrobium kibberense W25 according to the weight ratio of 0.2%, stir evenly and then let it stand in the magnetic field. The flocs of degrading bacteria are separated.

[0017] The maximum settling rate of the flocs can reach 1.4mm / s in the treatment of adding magnetic flocculants, and the density of bacteria in the flocs can reach 1.7×10 after 10 minutes 9 Cfu / ml is 4.6 times higher than that of the fermentat...

Embodiment 2

[0019] The difference from Example 1 is that: the weight ratio of flocculant and magnetic seed in the magnetic flocculant is 10:2; the weight ratio of polyaluminum chloride and polyferric sulfate is 10:1.

[0020] The above-mentioned magnetic flocculant was added to the fermentation broth rich in petroleum hydrocarbon degrading bacteria Ornithinimicrobium kibberense W25 according to the weight ratio of 0.3%.

[0021] The maximum sedimentation rate of the flocs can reach 1.5mm / s in the treatment of adding magnetic flocculants, and the density of bacteria in the flocs can reach 1.9×10 after 10 minutes 9 Cfu / ml is 5.1 times higher than that of the fermentation broth without flocculation treatment, and 4.9 times higher than the traditional process of only adding polyferric sulfate flocculation.

[0022] Except for the above content, the remaining content of this embodiment is the same as that of Embodiment 1.

Embodiment 3

[0024] The difference from Example 1 is that the weight ratio of flocculant and magnetic seed in the magnetic flocculant is 100:5; the weight ratio of polyaluminum chloride and polyferric sulfate is 1:10.

[0025] The above-mentioned magnetic flocculant was added to the fermentation broth rich in petroleum hydrocarbon degrading bacteria Ornithinimicrobiumkibberense W25 according to the weight ratio of 0.1%.

[0026] The maximum sedimentation rate of the flocs can reach 1mm / s in the treatment of adding magnetic flocculants, and the density of bacteria in the flocs can reach 1.3×10 after 10 minutes 9 cfu / ml, which is 3.4 times higher than that of the fermentation broth without flocculation treatment, and 3.2 times higher than the traditional process of only adding polyferric sulfate flocculation.

[0027] Except for the above content, the remaining content of this embodiment is the same as that of Embodiment 1.

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PUM

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Abstract

The invention discloses a magnetic flocculant which consists of a flocculant and a magnetic seed, wherein the weight ratio of the flocculant to the magnetic seed is (1-100):(1-5). The invention also discloses an application method of the magnetic flocculant, which comprises the following steps of: adding the magnetic flocculant into fermentation liquid rich in petroleum hydrocarbon degrading thalli according to a weight ratio of 0.1-0.5%; uniformly stirring, and standing in a magnetic field; and after the substance to be shaped is condensed, separating out the flocculating body rich in petroleum hydrocarbon degrading thalli by use of separation equipment. According to the magnetic flocculant and the application method thereof disclosed by the invention, by taking the fermentation engineering as a theoretical basis and combining the related theoretical knowledge of the magnetic separation science and the environmental engineering, the fermentation liquid thalli are recovered by use of an Fe3O4-containing magnetic flocculant to solve the problem in flocculation recovery of the fermentation liquid thalli in the traditional technology; and the magnetic flocculant and the application method thereof have the advantages that the flocculation recovery is fast, the magnetic flocculant brings little influence on the activity of the microorganism thalli, and the magnetic seed in the magnetic flocculant can be repeatedly recovered and used.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a magnetic flocculant and an application method thereof. Background technique [0002] Obtaining a large number of petroleum hydrocarbon-degrading bacteria by microbial liquid fermentation is one of the key technologies for preparing petroleum hydrocarbon-degrading bacterial agents. A large amount of bacteria can be effectively obtained by increasing the density of bacteria in the fermentation broth and improving its recovery rate, so as to facilitate the subsequent preparation of bacterial agents. By optimizing and controlling the fermentation medium and fermentation conditions, the cell density of the fermentation broth can be effectively increased. There are many ways to improve the recovery rate of fermentation broth cells, mainly including: centrifugal method, spray drying method, plate and frame filter press method and flocculation method. Among...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C12R1/01
Inventor 王新新吴亮杨勇安伟牛志刚丁东华
Owner CHINA NAT OFFSHORE OIL CORP