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Kit for early diagnosis of cryptococcus infections

A technology for early diagnosis and cryptococcosis, which is applied in the directions of microorganism-based methods, microbial determination/examination, biochemical equipment and methods, etc. problem, to achieve the effect of strong operability, long time period for solving diagnosis, and convenient interpretation of results

Active Publication Date: 2013-09-04
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In summary, based on DNA sequence analysis, try to find a rapid, simple, operable, and easy-to-interpret method for the rapid and early diagnosis of cryptococcal infection, which will help solve the problem of long diagnosis period of cryptococcal infection in clinical practice. It is of great significance to issues such as high requirements for testing personnel's technology and laboratory platform conditions, and will provide convenience for early diagnosis and timely treatment in clinical practice

Method used

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  • Kit for early diagnosis of cryptococcus infections
  • Kit for early diagnosis of cryptococcus infections
  • Kit for early diagnosis of cryptococcus infections

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Screening of primers for early diagnosis of cryptococcal infection and verification of specificity and sensitivity

[0040] 1. Main experimental materials and equipment

[0041] 1 strain

[0042] The standard strains CBS10083 (WM161), CBS10079 (WM629), CBS10078 (WM179), CBS10085 (WM148), CBS10080 (WM628), CBS10082 (WM178), CBS10083 ( WM626), CBS10101 (WM779) (purchased from the CBS International Fungal Diversity Research Center in the Netherlands), H99, JEC20 and 41 standard strains and 34 clinical strains such as Candida used to verify the specificity of the LAMP method, from Shanghai Medicine The Key Laboratory of Fungal Molecular Biology has a total of 75 strains.

[0043] 2 equipment

[0044] Ultra-clean workbench: CA-1390-1 vertical laminar flow clean workbench, Shanghai Shangjing Purification Equipment Co., Ltd.; biological safety cabinet: NUAIRE Biological Safefy Cabinet Class Ⅱ; water-insulating electric heating constant temperature incubator: Heraeus B5060 E...

Embodiment 2

[0164] Example 2 Kit 1 of the present invention

[0165] The kit contains the following reagents and materials:

[0166] Primer solution: IGS-1-F3 (SEQ ID NO.1, 5μmol / L); IGS-1-B3 (SEQ ID NO.2, 5μmol / L); IGS-1-BIP (SEQ ID NO.3, 40μmol) / L); IGS-1-FIP (SEQ ID NO.4, 40μmol / L).

Embodiment 3

[0167] Example 3 Kit 2 of the present invention

[0168] The kit contains the following reagents and materials:

[0169] Primer solution: IGS-2-F3 (SEQ ID NO.5, 5μmol / L); IGS-2-B3 (SEQ ID NO.6, 5μmol / L); IGS-2-BIP (SEQ ID NO.7, 40μmol) / L); IGS-2-FIP (SEQ ID NO.8, 40μmol / L).

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Abstract

The invention relates to a primer pair for early diagnosis of cryptococcus infections and a kit containing the same. The primers are designed by using an IGS1 sequence of the most common clinical strain of newborn cryptococcus in the world-H99 strain as a target sequence by utilizing LAMP (loop-mediated isothermal amplification) professional primer design software. The experiment on effectiveness, specificity and sensitivity proves that the primers can be used for early diagnosis of cryptococcus infections, have the advantages of high speed, simpleness, strong operability and convenient result identification, solve the problems of long time period for diagnosing cryptococcus infections, low requirements for detection personnel technique and laboratory platform conditions and the like, can be made into a kit, and provide convenience for early diagnosis and timely treatment of cryptococcus infections.

Description

technical field [0001] The invention relates to the technical field of molecular detection, in particular to a kit for early diagnosis of cryptococcal infection. Background technique [0002] Invasive fungal diseases (IFDs) refer to systemic infectious diseases caused by pathogenic fungi invading heart, liver, spleen, lung, kidney, brain, blood and other internal organs. In the past two decades, due to the widespread use of immunosuppressants, various intubation techniques and the continuous spread of HIV, the morbidity and mortality of IFDs have continued to rise globally. The data from the China Hospital Infection Surveillance Network shows that IFDs It has become the second important component of nosocomial infection in my country (accounting for about 24.1%), among which Candida albicans ( Candida albicans ), Aspergillus fumigatus ( Aspergillus fumigatus ), Cryptococcus neoformans ( Cryptococcus neoformans ) are the top three pathogenic fungi of invasive fungal disea...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04C12N15/11C12R1/645
Inventor 陈敏李梦潘炜华廖万清
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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