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Application of food-grade microorganism in degradation of zearalenone

A zearalenone and microorganism technology, applied in the field of microorganisms, can solve the problems of low degradation rate, time-consuming and laborious organic solvents, high cost, and achieve the effects of high degradation efficiency, short degradation period and product safety.

Inactive Publication Date: 2013-09-11
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional physical and chemical techniques for degrading zearalenone have many disadvantages, such as poor degradation effect, low degradation rate, high cost, time-consuming and laborious, and residual organic solvents, etc.

Method used

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  • Application of food-grade microorganism in degradation of zearalenone
  • Application of food-grade microorganism in degradation of zearalenone
  • Application of food-grade microorganism in degradation of zearalenone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Bacillus subtilis 168 and Bacillus natto CICC24640 were selected as the experimental strains, and appropriate medium and culture conditions were selected for inoculation and activation.

[0028] (2) Take 4 mL of corn oil, soybean oil, beer and milk, and add ZEN to make liquid food with a final concentration of ZEN of 0.5 μg / mL as the degradation matrix.

[0029] (3) Take Bacillus subtilis 168 and Bacillus natto CICC246403g respectively, and inactivate them by high-pressure steam (121°C, 20min). The inactivated Bacillus subtilis 168 and Bacillus natto CICC24640 were inoculated in the aforementioned strain degradation matrix. The samples were incubated under anaerobic conditions at 30°C for 1 h. The obtained suspension was centrifuged at 10000rpm, 4°C for 10min. The supernatant was collected and ZEN was extracted with a mixture of chloroform-methanol (9:1 v / v) for detection. The adsorption rates of ZEN in different liquid food matrices adsorbed by two kinds of inac...

Embodiment 2

[0035] (1) Bacillus subtilis 168 and Bacillus natto CICC24640 were selected as the experimental strains, and appropriate medium and culture conditions were selected for inoculation and activation.

[0036] (2) Soybean, flour and corn flour were selected as solid substrates, and the moisture content and pH value of the samples were adjusted to 30% and 7, respectively. The solid substrate was stirred to form a paste, followed by autoclaving at 121° C. for 20 minutes.

[0037] (3) Add ZEN to the sample base to make the final concentration 1mg / kg, and then inoculate 1mL of the overnight cultured strain (10 8 cfu / mL), all samples were performed in duplicate. The samples were incubated in a 37°C incubator after mixing. After 0h, 6h, 12h, 18h, 24h, 30h, 36h, 42h, and 48h, take out a certain amount and add chloroform-methanol (9:1, v / v) mixture to extract zearalenone, and use UPLC-DAD The ZEN in the sample is determined. Table 3 shows the degradation rates of ZEN in different soli...

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Abstract

The invention relates to application of a food-grade microorganism in degradation of zearalenone. Probiotics is any one or more of bacillus subtilis 168, lactic acid bacteria NYC30, lactic acid bacteria Dins 37, acetobacter aceti sp and bacillus natto CICC24640; further, the probiotics is preferably the bacillus subtilis 168 and bacillus natto CICC24640. The application has the advantages that the food-grade probiotics strain is adopted and used for degrading fungaltoxin zearalenone, so that the degradation period is short, the degradation efficiency is high, the product is safe, obvious advantages are brought for the safety of foods for people and livestock, and a new way is provided for the degradation of zearalenone.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to the application of a food-grade microbial degradation strain to degrade the mycotoxin zearalenone in food or feed. Background technique [0002] Zearalenone (zearalenone, ZEN) is a non-steroidal mycotoxin, its chemical name is 6-(10-hydroxy-6-oxyl-carbenyl) β-rhycloic acid-μ-lactone , also known as F-2 toxin. The toxin-producing bacteria are mainly strains of the genus Fusarium, such as Fusarium graminearum, Fusarium three-line and Fusarium nivalum, etc., which widely exist in moldy corn, wheat, rice and other cereal crops and milk. ZEN is a substituted 2,4-dihydroxybenzoic acid lactone with estrogenic activity. At present, at least 15 kinds of derivatives of ZEN have been found, and the metabolic pathway is mainly through the combination with glucuronic acid, and the reduction to Zearalenol (Zearalenol, ZEL), and ZEL has two non-stereoisomers, namely α-ZEL and β-ZEL. Both have ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/015A23L5/20
Inventor 谢云飞范雨豪汪何雅姚卫蓉钱和
Owner JIANGNAN UNIV
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