A holographic fluorescent molecular imaging system and method

Abstract

The invention relates to a holographic fluorescent molecular imaging system and a method. The holographic fluorescent molecular imaging system comprises a testing platform used for containing an object to be imaged, an exciting light lighting unit arranged on one side of the testing platform, a CCD detecting device arranged on the other side of the testing platform and a computer connecting the exciting light lighting unit and the CCD detecting device. The holographic fluorescent molecular imaging system is characterized in that a holographic fluorescence wave filtering unit is arranged between the testing platform and the CCD detecting device. According to the holographic fluorescent molecular imaging system and the method, the holographic fluorescence wave filtering unit serves as a device for spatial wave filtering and spectrum wave filtering, so that the CCD detecting device can simultaneously and directly obtain a spatial distribution image and rich multispectral fluorescence information of a two-dimension fluorescence indicator or a three-dimension fluorescence indicator. Therefore, a complicated image reconstruction algorithm and time-consuming complicated scanning are not needed.

Description

technical field [0001] The invention relates to a fluorescent molecular imaging system and method, in particular to a holographic fluorescent molecular imaging system and method using a holographic grating as a spatial filter and / or spectral filter. Background technique [0002] Fluorescence molecular imaging technology is an emerging molecular imaging technology that has developed rapidly in recent years, and has broad application prospects in the fields of tumor detection, drug development, and disease diagnosis. Fluorescent molecular imaging technology uses fluorescent markers to mark specific molecules or cells in small animals, and when the labeled small animals are irradiated with excitation light of appropriate wavelength and intensity, the fluorescent markers in the small animals are excited to emit fluorescence. A certain device detects the fluorescence intensity, and the distribution image of the fluorescence optical properties inside the tissue can be obtained, so...

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Problems solved by technology

[0003] However, the fluorescence filtering of existing fluorescent molecular imaging systems mostly uses spectral dispersion filter devices such as ordinary optical filters and liquid crystal tunable filters (LCTF), and only two-dimensional, single-spectrum fluorescent markers can be obtained in a single signal acquisition. Information

This makes it necessary to perform multiple scans and use complex image reconstruction algorithms when the system needs to reconstruct a three-dimensional, multi-spectral fluorescence image, which results in long time-consuming, poor time resolution and spectral bands of the existing fluorescent molecular imaging system. few

In practical applications, it is often necessary to achieve fast spatial, spectral, and temporal signal acquisition of fluorescent signals, especially for some fast, dynamically changing drug metabolism and imaging analysis of multi-fluorescent markers. Existing fluorescent molecular imaging The system cannot meet the requirements

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