Unlock instant, AI-driven research and patent intelligence for your innovation.

Construction method of SSR fingerprint suitable for identification of rice hybrid authenticity

A fingerprint, hybrid technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of simple, fast and accurate identification of the authenticity of hybrids not suitable for crops, and achieve stable results and simple operation. , detect fast effects

Inactive Publication Date: 2013-09-11
ZHEJIANG UNIV
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Conventional SSR fingerprinting methods are mostly based on agarose or polyacrylamide gel electrophoresis patterns of SSR amplification products, which are not suitable for the simple, rapid and accurate identification of the authenticity of crop hybrids

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Construction method of SSR fingerprint suitable for identification of rice hybrid authenticity
  • Construction method of SSR fingerprint suitable for identification of rice hybrid authenticity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0026] In this example, the rice hybrid Y Liangyou 689 and its female parent Y58S and male parent R689 seeds were used as test materials, and the seedling genomic DNA was extracted as a template. SSR primer RM211 was used for SSR-PCR amplification, and the SSR-PCR product was high. Resolution melting curve analysis, using GeneScan software for data analysis, and using the constructed SSR fingerprint map for authenticity identification of rice hybrid Y Liangyou 689.

[0027] The specific steps are as follows:

[0028] (1) Genomic DNA extraction

[0029] Using rice hybrids and their parent seeds as materials, young leaves were taken at the seedling stage to extract DNA by CTAB method, and a portion of the DNA solution was taken to measure its concentration, diluted to 10ng / μL, and stored at -20°C for later use.

[0030] (2) SSR-PCR amplification and high-resolution melting curve analysis

[0031] Using 10μL reaction system, containing Mg 2+ (25mM) 1μL, SSR primer RM211 upstream and downs...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a construction method of an SSR fingerprint suitable for identification of rice hybrid authenticity. The method sequentially comprises the following steps of: subjecting a rice hybrid and its parental seeds to genomic DNA extraction, carrying out SSR-PCR amplification and high resolution melting curve analysis on the genomic DNA, and utilizing GeneScan software to perform SSR fingerprint construction based on the SSR-PCR amplification and high resolution melting curve analysis results. Compared with routine planting test and biochemical test, construction of the fingerprint of the rice hybrid and its parents by the SSR molecular marker technology is not affected by the crop growth environment and season, the result is more stable and reliable, and time-saving and labor-saving effects can be achieved. Compared with conventional gel electrophoresis and capillary electrophoresis detection, the method is safer, cheaper, fast and visualized.

Description

Technical field [0001] The invention relates to a hybrid authenticity identification technology in biotechnology, in particular to an SSR fingerprint map construction method suitable for rapid and accurate identification of the authenticity of rice hybrids. Background technique [0002] Rice is an important food crop. my country is the world's largest producer and consumer of hybrid rice seeds and has a huge hybrid rice seed market. The authenticity and purity of rice hybrids are important indicators to measure the quality of seeds, which directly affect the yield and quality of crops and the interests of farmers. Fast, accurate, simple and economical identification of the authenticity of hybrid rice seeds is of great significance to breeding and agricultural production. The traditional morphological identification method relies on the phenotypic differences of varieties, and the performance of morphological traits is greatly affected by the environment, and the morphological id...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 胡晋朱岩芳关亚静王建成胡伟民祝水金
Owner ZHEJIANG UNIV