Application of anti-heat shock protein 70 (HSP70) in preventing and/or treating hypertensive heart disease and heart failure
A high blood pressure and heart disease technology, applied in the direction of antibodies, cardiovascular system diseases, drug combinations, etc., can solve the problem of no more effective treatment for severe heart failure, and achieve the effect of significant curative effect and safe use.
Inactive Publication Date: 2013-09-18
INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
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Problems solved by technology
At present, although great progress has been made in anti-heart failure drug therapy, interventional therapy, and surgery, these treatments can only improve the symptoms and signs of heart failure patients, improve their quality of life, and delay the progression of the disease. Heart failure no more effective treatment than heart transplant
Method used
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Embodiment 1
[0049] 1. Experimental main reagents and experimental animals
[0050] C57BL / 6J mice were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. (male, 8-10 weeks, 18-23g). All animals were kept in the Experimental Animal Center of Institute of Materia Medica, Chinese Academy of Medical Sciences, with constant temperature and humidity, and free diet.
[0051] Doxorubicin (Dox) was purchased from Shenzhen Wanle Pharmaceutical Co., Ltd.; anti-IgG antibody was purchased from Biolegend; anti-HSP70 antibody was purchased from Santa Cruz; anti-α-SMA antibody was purchased from Boster; Western Blot The DAB chromogenic reagent used in the method was purchased from Beijing Pulilai Biotechnology Co., Ltd.; the hematoxylin staining solution, eosin reagent and Masson trichrome staining kit were purchased from Fuzhou Maixin Biotechnology Development Co., Ltd.
[0052] 2 Experimental methods
[0053] 2.1 Preparation and group design of mouse abdominal aortic coarcta...
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The invention relates to an application of an anti-heat shock protein 70 (HSP70) in preventing and / or treating hypertensive heart disease and heart failure. An anti-HSP70 neutralizing antibody (Anti-HSP70Ab) of the HSP70 is utilized to inhibit the activity of an extracellular HSP70 molecule on a mouse hypertensive heart disease model, a subacute heart failure model and a chronic hear failure model, so that the animal survival rate can be obviously increased, and cardiac dysfunctions can be partially converted; the HSP70 can be used for inhibiting myocardial hypertrophy caused by hypertension and myocardial fibrosis caused by various reasons, and reducing collagen deposition and alpha-sooth muscle actin (SMA) expression; the HSP70 has functions of regulating the heart local immune microenviroment, inhibiting the inflammatory response and enabling inflammatory reaction to shift towards active inflammation outcome. Results prove that the HSP70 can inhibit the extracellular HSP70 molecular activity and has an obvious treatment function on hypertensive heart disease and heart failure.
Description
technical field [0001] The invention relates to the application of inhibiting the activity of extracellular heat shock protein 70 in the preparation of medicines for treating hypertensive heart disease and heart failure, and belongs to the technical field of medicine. Background technique [0002] Congestive or chronic heart failure, referred to as heart failure, is the end stage of heart disease caused by various reasons. The systolic and (or) diastolic function of the heart is significantly reduced, so that the cardiac output cannot meet the metabolic needs of the body. There are many causes of it, mainly including coronary heart disease, hypertension, cardiomyopathy, cardiotoxin, valvular disease and congenital heart disease. According to epidemiological statistics, the prevalence of heart failure is as high as 1-2%, 20% of patients die within 1 year after diagnosis, and the mortality rate is higher than 50% within 5 years. Hundreds of thousands of people die of heart fai...
Claims
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Login to View More IPC IPC(8): A61K39/395A61P9/04A61P9/12
Inventor 胡卓伟鲍华燕刘鹏蔡文锋
Owner INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI