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Application of catalpol and retinoic acid medicine composition in preparing medicine used for preventing or treating white matter damage

A technology of brain white matter injury and composition, applied in the field of medicine, can solve the problems of lack of safe and effective treatment methods, and achieve the effect of application safety

Inactive Publication Date: 2013-09-25
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there is still a lack of safe and effective treatments for this type of disease

Method used

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  • Application of catalpol and retinoic acid medicine composition in preparing medicine used for preventing or treating white matter damage
  • Application of catalpol and retinoic acid medicine composition in preparing medicine used for preventing or treating white matter damage
  • Application of catalpol and retinoic acid medicine composition in preparing medicine used for preventing or treating white matter damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] The pharmaceutical composition of embodiment 1CAT-RA

[0074] For the pharmaceutical composition of CAT-RA, 100 mg of catalpol and 0.3 mg of retinoic acid are taken, and the molar ratio of catalpol to retinoic acid is 280:1, dissolved in physiological saline to 100 ml.

Embodiment 2

[0075] The pharmaceutical composition of embodiment 2CAT-RA

[0076] For the pharmaceutical composition of CAT-RA, 500 mg of catalpol and 0.45 mg of retinoic acid are taken, and the molar ratio of catalpol to retinoic acid is 1380:1.5, dissolved in physiological saline to 100 ml.

[0077] The effect of different concentrations of CAT-RA on cell viability is bidirectional. When the concentration of catalpol in CAT-RA is lower than 5mM, cell survival can be promoted; as the concentration of catalpol in CAT-RA is higher than 5mM, As the concentration increased, the cell viability decreased, indicating that high concentrations of catalpol can have a toxic effect on cells. Before carrying out the animal model experiment research, according to the experimental results combined with the relevant literature reports, the dosage of catalpol below 10mg / kg is its safe dosage.

Embodiment 3

[0078] Example 3 Effect of CAT-RA on pathological changes of white matter in rats

[0079] Adult male Wistar rats were divided into normal group, ischemic group and treatment group. Catalpol and retinoic acid were dissolved in normal saline. The ischemia group is a chronic cerebral ischemia model established by permanent ligation of bilateral common carotid arteries in rats, and 5 mg / kg normal saline is injected intraperitoneally after operation. In the treatment group, the CAT-RA of Example 1 was injected intraperitoneally after permanent ligation of the bilateral common carotid arteries of the rats, and the administration dose was 1 mg / kg of catalpol, once a day for 10 consecutive days. All animals were sacrificed 30 days after operation. Paraffin sections (5 μm in thickness) and H&E staining were performed on brain tissue blocks containing the corpus callosum. The vacuolar area of ​​the corpus callosum was measured under a microscope.

[0080] The result is as Figure 6 ...

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Abstract

The invention belongs to the field of medicine, and relates to the field of medicine preparation treatment activity. The invention specifically relates to a catalpol and retinoic acid medicine composition CAT-RA and an application thereof in preparing medicines used for preventing and treating with white matter damage after chronic cerebral ischemia. As a result of experiments of CAT-RA in cells and in chronic cerebral ischemia disease animal models, CAT-RA assists in reducing brain tissue inflammatory responses after brain damage, reducing white matter pathological changes, inhibiting oligodendrocyte apoptosis and myelin damage in white matters, and promoting oligodendrocyte precursor cell differentiation and maturation. CAT-RA has the effects of protecting white matters and promoting white matter remyelination. Therefore, CAT-RA can be used as an active component for preparing medicines used for preventing and treating white matter damage after chronic cerebral ischemia, and can also be used for preparing medicines used for treating other cerebral vascular related diseases characterized in white matter damages. The invention provides a novel solution for prevention and treatment of white matter damage related diseases.

Description

technical field [0001] The invention belongs to the field of medicine and relates to the field of therapeutic activity of pharmaceutical preparations, in particular to the pharmaceutical composition CAT-RA of catalpol and retinoic acid and its application in the preparation of medicines for preventing or treating chronic cerebral white matter damage. Background technique [0002] In the central nervous system, where the neuron cell bodies and dendrites accumulate, the fresh specimens are dark in color due to rich blood vessels, which is called gray matter; where the nerve fibers gather, the myelin sheath is bright and white, called white matter, deep in the inner cortex The white matter is also called the medulla. The white matter of the brain is mainly composed of nerve fibers and glial cells. The myelin sheath of nerve fibers in the central nervous system is formed by oligodendrocytes. The white matter of the adult brain accounts for about 50% of the total brain volume, an...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P25/00A61P29/00A61K31/203
Inventor 李红丽蔡其燕肖岚吴锡艳田衍平李成仁
Owner ARMY MEDICAL UNIV
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