Manganese oxidizing bacteria and application thereof

A technology of manganese oxidation and bacteria, which is applied in the direction of bacteria, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of nutrient deficient microorganisms, affect the survival of bacterial strains and manganese oxidation activity, and achieve the effect of broad application prospects

Active Publication Date: 2013-10-02
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Under natural water quality conditions, there are nutrient deficiencies and microbial competition, which will affect the survival and manganese oxidation activity of the strains

Method used

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  • Manganese oxidizing bacteria and application thereof
  • Manganese oxidizing bacteria and application thereof
  • Manganese oxidizing bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1. Isolation, purification and identification of Pseudomonas putida QJX-1

[0047] 1. Isolation and purification of Pseudomonas putida QJX-1

[0048] Pseudomonas putida QJX-1 was obtained from the manganese ore piled soil in Xiangtan, Hunan, after domestication, separation and purification. Pseudomonas putida QJX-1 is a Gram-negative bacterium. Specific steps are as follows:

[0049] Take the soil where manganese ore is piled up in Hunan Xiangtan Manganese Mine, add 2g of soil to PYG (peptone-yeast extract-glucose) medium (peptone-yeast extract-glucose) medium (peptone, glucose, yeast extract each 0.25g / L, CaCl 2 .2H 2 O content 8mg / L, MgSO 4 .7H 2 O content 0.5g / L, MnCl 2 Content 100μM, deionized water 1L), the buffer in the medium is HEPES (N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid, 4-hydroxyethylpiperazineethanesulfonic acid) with a final concentration of 10-20mM , pH7.5. where MnCl 2 The solution and HEPES buffer were sterilized and adde...

Embodiment 2

[0062] Embodiment 2, Pseudomonasputida QJX-1 bacterial strain is to Mn 2+ Study on the biological oxidation characteristics of

[0063] 1. Pseudomonas putida QJX-1 strain on Mn 2+ biological oxidation

[0064] After adding Mn 2+ In the PYG medium of the present invention, investigate the manganese oxidizing bacteria (Pseudomonas putida QJX-1, preservation number: CGMCC No.6630) of the present invention to Mn 2+ oxidation capacity. It is found that the bacteria of the present invention are sensitive to Mn 2+ It has good oxidation ability and can convert Mn 2+ All are oxidized to manganese oxides which are insoluble in water. Specific steps are as follows:

[0065] 1.1) Get the bacterium liquid of 2mL manganese oxidizing bacteria of the present invention, inoculate to 100mL does not contain Mn 2+In the Erlenmeyer flask of the PYG medium, 30 ° C, 170 rpm shaking culture for 48 hours.

[0066] 1.2) Take 10 mL of the cultured bacterium solution described in step 1.1) for ...

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Abstract

The invention discloses manganese oxidizing bacteria capable of oxidizing Mn<2+>. The bacteria can oxide the Mn<2+> in a water body to generate water-insoluble manganese oxide. The Mn<2+> oxidizing bacteria are a pseudomonas putida QJX 1 strain with a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.6630. The pseudomonas putida QJX 1 strain provided by the invention is used for oxidizing the Mn<2+> in natural water bodies, wherein the operating temperature (10-30 DEG C) is within a normal temperature range, the pH value (6.5-8.5) is within a neutral range, and the pseudomonas putida QJX 1 strain has high Mn<2+> oxidation efficiency. The invention also relates to a method for removing Mn<2+> in water bodies or solid matrixes. The method comprises the following steps: inoculating the manganese oxidizing bacteria or a microbial agent containing the manganese oxidizing bacteria into the water bodies or solid matrixes, and culturing for a proper time under conditions of 10-35 DEG C and pH of 6.5-8.5.

Description

technical field [0001] The invention relates to a manganese oxidizing bacterium and its enhanced application in water. Specifically, the manganese oxidizing bacteria is Pseudomonas putida QJX-1, and its preservation number is: CGMCC No.6630. Background technique [0002] Manganese is an abundant metal element in the earth's crust, Mn 2+ It is one of the essential trace elements for the body, but drinking water with excessive manganese for a long time will cause physiological diseases in the human body. Manganese in water can have from Mn 2+ to Mn 7+ various valence states, except for Mn 2+ and Mn 4+ In addition, manganese in other valence states is generally unstable in natural water with a neutral pH. in Mn 2+ and Mn 4+ Medium, Mn 4+ The solubility in natural water is very low, not harmful; therefore, in natural groundwater, manganese in dissolved state is mainly Mn 2+ . However, the manganese content in groundwater in many areas of our country exceeds the nation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/40
Inventor 曲久辉柏耀辉周娜娜梁金松刘锐平胡承志
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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