Bacterium for degrading herbicide acifluorfen-sodium and application of bacterium

A technology of weed incineration and fungicide, applied in the field of bacteria for degrading herbicide weed incineration, can solve the problems of slow natural degradation, toxicity, short half-life, etc.

Active Publication Date: 2013-10-02
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extensive use of herbicides has greatly saved labor costs and improved agricultural production efficiency, but it has also brought some ecological problems such as agricultural production and environmental pollution.
Weed incineration has a short half-life and is generally considered a low-toxic, environmentally friendly pesticide. However, in recent years, frequent, excessive or improper use has caused varying degrees of pollution to farmland soil and groundwate

Method used

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  • Bacterium for degrading herbicide acifluorfen-sodium and application of bacterium
  • Bacterium for degrading herbicide acifluorfen-sodium and application of bacterium
  • Bacterium for degrading herbicide acifluorfen-sodium and application of bacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1, Isolation and Identification of Enterobacter sp. ZCF1643CGMCC No.7774

[0038] 1. Isolation of weed incinerator degrading bacteria ZCF1643

[0039] Add 10 g of soil samples (collected from farmland polluted by herbicide weed incineration in Shaanxi Province, China) to 100 mL of enrichment medium containing 200 mg / L of weed incineration, and culture at 28°C and 200 r / min for 7 days to enrich in weed incineration. degrading bacteria. After the first round of enrichment, 10ml of the enrichment solution was transferred to 100ml of the enrichment medium containing 400mg / L of weed fungus, and the culture was continued for 7 days for the second round of enrichment. Such continuous enrichment and culture for 5 times, the weed concentration was 200, 400, 600, 800 and 1000 mg / L in turn. After the enrichment is completed, the enrichment solution is evenly spread on the inorganic salt medium containing 1000 mg / L of weeds, and cultured at a constant temperature of 28°C ...

Embodiment 2

[0061] Example 2, Quantitative Determination of Enterobacter sp. ZCF1643CGMCC No.7774 Degradation Weed Burning Ability

[0062] 1. Preparation of standard curve for weed burning determination

[0063] Accurately weigh 10.0 mg of the weed incineration standard (purchased from Fluka) into a 10 mL volumetric flask, dissolve it with a small amount of methanol, place the volumetric flask in an ultrasonic bath and vibrate for 10 min, then dilute to 10 mL with methanol, shake well, and prepare Become 1000mg / L weed burning mother liquor. Prepare 10, 20, 40, 60, 80mg / L series of standard solutions. High performance liquid chromatography (HPLC) was used to measure the peak areas of different concentrations of weed incineration standard samples, with 3 repetitions. With the weed incineration concentration as the abscissa and the peak area as the ordinate, draw the weed incineration standard curve.

[0064] The detection conditions are as follows:

[0065] Detection system: Agilent110...

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Abstract

The invention discloses a bacterium for degrading a herbicide acifluorfen-sodium and application of the bacterium. The bacterium for degrading acifluorfen-sodium is Enterobacter sp. ZCF1643, which is preserved in China General Microbiological Culture Collection Center by a preservation number of CGMCC No.7774. The enterobacter sp. ZCF1643 CGMCC No.7774 reaches a degrading rate of 63.47% on 25mg/L of acifluorfen-sodium in an organic salt culture medium within 7 days, showing that the strain can effectively degrade the acifluorfen-sodium; the bacterium has a wide application prospect in an aspect of repairing acifluorfen-sodium pollution of soil.

Description

technical field [0001] The invention relates to a bacterium for degrading herbicide weed burn and its application. Background technique [0002] Weed grass fire, also known as acifluorfen, English name acifluorfen, chemical name 5-(2-chloro-α,α,α-trifluoro-p-tolyloxy) 2-nitrobenzoic acid, the structure is as shown in formula 1 Show: [0003] [0004] Weed burning is a diphenyl ether herbicide, a contact herbicide, and a protoporphyrin oxidase inhibitor. Usually, 25% water agent is used for pre-emergence surface soil treatment of soybeans and post-emergence spraying through leaves, which can effectively control broad-leaved weeds in soybean fields such as cocklebur, velvetleaf, nightshade, amaranth, quinoa, polygonum, morning glory, ragweed, mangosteen, etc. Tuola, purslane, iron amaranth, ghost needle grass, weasel petal flower, commelina, etc., early post-emergence treatment can also control annual grass weeds including foxtail. After spraying, soybeans will be damage...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02B09C1/10C12R1/01A62D101/04A62D101/28
Inventor 孙建光张颖张静胡海燕
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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