Sheep embryonic cell culture fluid

An embryonic cell and culture medium technology, applied in the field of sheep embryo cell culture medium, can solve the problems of restraint transgenic animal research technology, low rate of transplant conception, poor quality, etc., to increase uterine receptivity, improve survival rate, and facilitate development. Effect

Active Publication Date: 2015-03-25
青岛森淼生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, early embryos are quite sensitive to environmental changes during in vitro culture. Compared with in vivo embryos, there are problems such as poor quality and low implantation and conception rate, which restricts the development of transgenic animal research technology.

Method used

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  • Sheep embryonic cell culture fluid
  • Sheep embryonic cell culture fluid

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Experimental program
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Embodiment Construction

[0011] The medium of the present invention will be described in detail below with reference to the examples.

[0012] 1. Preparation of fetal fluid:

[0013] The fetal fluid is prepared according to the existing method. First, B-ultrasound is performed on the pregnant sheep to determine the placenta position and avoid accidentally injuring the placenta. Select the needle entry point, disinfect the skin, and puncture the selected point vertically with a lumbar puncture needle. The needle passes through the abdominal wall and uterine wall, and the fetal fluid is extracted, and then put into sterile test tubes, capped, and set aside.

[0014] 2. Basic culture medium and additives

[0015] See Table 1 for the relevant components required for the preparation of the basal culture solution of the present invention. Take the required amount of each relevant component respectively, add to 1000ml double distilled water and mix well.

[0016] Prepare 5 μmol / L of Ionomycin, 2 mmol / L o...

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Abstract

The invention provides sheep embryonic cell culture fluid. The sheep embryonic cell culture fluid comprises the following components: calcium chloride dehydrate, anhydrous magnesium sulfate, potassium chloride, monopotassium phosphate, sodium bicarbonate, sodium chloride, bovine serum albumin, glucose, sodium pyruvate, sodium lactate and phenol red sodium salt. Embryonic fluid at a volume by concentration of 10% is also added to the culture fluid so that the culture fluid has a great cell culture effect. According to the culture fluid, the components collaborate with each other, so that the irritation to an early embryo is reduced, and the embryo can grow well; and in addition, 10% of uterine embryonic fluid of a ewe is added to the culture fluid, so that the fusion rate of a reconstructed embryo can be remarkably increased. Compared with the culture fluid without the embryonic fluid, the culture with the embryonic fluid has the capability of obviously increasing the survival rate and blastocyst rate of in-vitro cultured embryos; and the culture fluid provided by the invention also can improve uterine receptivity, therefore, the lambing percentage is increased obviously.

Description

technical field [0001] The invention belongs to the technical field of in vitro cell culture, and in particular relates to a sheep embryo cell culture solution. technical background [0002] Since the 1980s, research on transgenic animals has made significant progress in the fields of improving growth and development speed, nutritional content, resistance to environmental conditions, and environmental pollution indicators, especially in the production of pharmaceutical proteins by the mammary gland bioreactor technology of transgenic animals. The application in fields such as food and functional food demonstrates the strong application potential of transgenic animal research technology. [0003] Embryo culture in vitro is an important link in the field of transgenic animal research. The environment of culturing embryos in vitro directly affects the development of embryos and the birth rate after transplantation. A large number of long-term studies have shown that one of th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/073
Inventor 邹贤刚赵雅琳袁三平
Owner 青岛森淼生物技术有限公司
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