Gene for preventing toxoplasma infection and application of gene
An infection prevention, Toxoplasma gondii technology, applied in the fields of immunology and molecular biology, can solve the problems such as the nucleic acid vaccine against Toxoplasma gondii has not achieved a relatively ideal effect, the lymphocyte binding site is few, and the restriction is large, and the prevention and the effect of controlling infection and improving immunity
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[0034] The following examples facilitate a better understanding of the present invention, but do not limit the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the following examples, unless otherwise specified, were purchased from conventional biochemical reagent stores. Quantitative experiments in the following examples were all set up to repeat the experiments three times, and the results were averaged.
[0035] Female KM mice, about 6 weeks old, weighing 20±2g, were purchased from the Medical Experimental Center of Lanzhou University.
[0036] Host bacteria, Toxoplasma gondii RH, PRU strains, pVAX1 eukaryotic expression vector and other materials were all preserved and provided by the Laboratory of Parasite Functional Genomics, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences.
[0037] PrimeScript? One Step RT-PCR Kit Ver.2 (Cat. No.: RR055B...
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