Gene for preventing toxoplasma infection and application of gene

An infection prevention, Toxoplasma gondii technology, applied in the fields of immunology and molecular biology, can solve the problems such as the nucleic acid vaccine against Toxoplasma gondii has not achieved a relatively ideal effect, the lymphocyte binding site is few, and the restriction is large, and the prevention and the effect of controlling infection and improving immunity

Active Publication Date: 2013-10-02
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, DNA vaccines have been used against parasites such as: schistosomes, roundworms, especially in protozoa, including Plasmodium, Leishmania, Trypanosoma and other parasites It has been widely used in prevention and treatment, and the vaccine research against Toxoplasma infection has also entered a new stage. However, the research on nucleic acid vaccines against Toxoplasma gondii has not achieved satisfactory results.
The reason is that the life cycle of Toxoplasma gondii is complex, the morphology is diverse, the host range is wide, the immunogens of different anti...

Method used

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  • Gene for preventing toxoplasma infection and application of gene
  • Gene for preventing toxoplasma infection and application of gene
  • Gene for preventing toxoplasma infection and application of gene

Examples

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Embodiment Construction

[0034] The following examples facilitate a better understanding of the present invention, but do not limit the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the following examples, unless otherwise specified, were purchased from conventional biochemical reagent stores. Quantitative experiments in the following examples were all set up to repeat the experiments three times, and the results were averaged.

[0035] Female KM mice, about 6 weeks old, weighing 20±2g, were purchased from the Medical Experimental Center of Lanzhou University.

[0036] Host bacteria, Toxoplasma gondii RH, PRU strains, pVAX1 eukaryotic expression vector and other materials were all preserved and provided by the Laboratory of Parasite Functional Genomics, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences.

[0037] PrimeScript? One Step RT-PCR Kit Ver.2 (Cat. No.: RR055B...

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Abstract

The invention provides a nucleotide sequence for preventing toxoplasma infection. The nucleotide sequences selected from the followings are contained: 1) 724-1470th nucleotide sequences as shown in SEQ ID No.3, and/or 724-2472nd nucleotide sequences as shown in SEQ ID No.1; 2) nucleotide sequences complementary with the nucleotide sequences as shown in 1); and 3) nucleotide sequences which perform substituting, deletion and addition modification on one or a plurality of basic groups of the nucleotide sequences as shown in 1) or 2). The pVAX-CDPK (Calcium Dependent Protein Kinase)1 can effectively prevent and control the infection of a toxoplasma animal model (Kunming mice), and pVAX-IL21-IL15 not only can be independently used for effectively preventing and controlling the toxoplasma infection, but also can be used as a cytokines adjuvant for effectively improving the immune effect of a vaccine pVAX-CDPK1.

Description

technical field [0001] The invention relates to the fields of immunology and molecular biology, in particular to a recombinant plasmid used for the prevention of toxoplasma gondii infection. Background technique [0002] Toxoplasma gondii ( Toxoplasma gondii , T. gondii ) is an obligate intracellular parasitic opportunistic pathogenic protozoan belonging to the class Sporozoasida, order Eucoccidiida, and family Sarcocystidae. The resulting toxoplasmosis is distributed worldwide, and about 1 / 3 of the world's people are infected, seriously endangering the health of humans and animals. As an opportunistic infection agent, Toxoplasma gondii can cause severe organ damage in immunosuppressed or immunocompromised patients (such as AIDS patients, organ transplantation and malignant tumor patients, etc.). Toxoplasma gondii infection for the first time during pregnancy can cause miscarriage, stillbirth, and congenital defects or deformities in the fetus. As a zoonotic disease, th...

Claims

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Application Information

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IPC IPC(8): C12N15/24C12N15/57C12N15/63C12N9/64C07K14/54A61K48/00A61K39/39A61P33/02
Inventor 陈佳朱兴全徐民俊黄思扬李中原宋慧
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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