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Sequencing method of rice bacterial leaf blight activating transcription factor like effector gene

A technology of transcription activator and rice bacterial blight, which is applied in the direction of microorganism-based methods, microbial measurement/testing, biochemical equipment and methods, etc., can solve the problems of functional research and application of TAL effectors, TAL effector genes Problems such as family sequencing and gene cloning are inconvenient

Inactive Publication Date: 2013-10-23
GUANGXI UNIV
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Problems solved by technology

The structural characteristics of this type of gene and the multi-copy problem bring inconvenience to the sequencing and gene cloning of the TAL effector gene family, which cannot provide essential support for the functional research and application of TAL effector

Method used

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  • Sequencing method of rice bacterial leaf blight activating transcription factor like effector gene
  • Sequencing method of rice bacterial leaf blight activating transcription factor like effector gene
  • Sequencing method of rice bacterial leaf blight activating transcription factor like effector gene

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Embodiment Construction

[0027] The technical solutions of the present invention will be described in detail below in conjunction with the accompanying drawings and preferred embodiments. It should be understood that the following examples are only used to illustrate and explain the present invention, but not to limit the technical solution of the present invention.

[0028] The embodiment of the sequencing method of the rice bacterial blight TAL effector gene provided by the present invention comprises the following steps:

[0029] Step 1: Genomic DNA of Xanthomonas oryzae BAI3R and BAI3 containing avrBs3 / pthA family genes RCosmid (cosmid, referred to as plasmid) library plasmids were completely digested with restriction endonuclease BamHI, and the resulting fragments were separated by electrophoresis on 0.8% agarose gel (20 hours at 30V); 122 to 842 base fragments as probes ( 32 P marker), carry out Southern hybridization (classical test method of molecular biology) on the isolated DNA fragments, ...

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Abstract

The invention provides a sequencing method of rice bacterial leaf blight activating transcription factor like effector gene, can effectively separate repeating region of nearly each TAL effector family gene, performs accurate sequencing and assembly to the TAL effector family gene, and provides essential support for the functional research and application of TAL effectors.

Description

technical field [0001] The invention relates to a sequencing method for a transcription activator-like (TAL, Transcription Activator-Like) effector gene of rice bacterial blight. Background technique [0002] Xanthomonas oryzae pv.oryzae (Xoo for short) is not only an important pathogen of rice, but also one of the model bacteria to study the interaction mechanism between bacteria and plants. The TAL effector of the bacteria, also known as AvrBs3 / PthA family protein, is the main pathogenic and sensitizing factor. The biggest feature of this type of protein is that it can specifically recognize DNA sequences, thereby activating or inhibiting the expression of related genes. [0003] AvrBs3 / PthA family proteins are a class of biomacromolecules with a high degree of evolution. The amino acid sequence homology among family members reaches 80%-97%. They also have many common features in structure, such as figure 1 As shown, the middle region (ie repeat region) is almost the sam...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12R1/64
Inventor 玉延华唐纪良冯家勋何勇强姜伯乐姜伟牛祥娜
Owner GUANGXI UNIV
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