Artificial mixed bait for portunus trituberculatus and preparation method thereof
A technology of portunus trituberculatus and bait, which is applied in the field of marine fishing, can solve the problems of large fish natural resources, damage to marine ecology, increase production costs, etc., and achieve the effects of reducing the consumption of natural resources, avoiding competition for food, and reducing use
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[0031] (1) Preparation of the enzymatic hydrolyzate of Qingzhan fish: remove the head, bones, viscera and fins of Qingzhan fish, wash and drain, and crush it with a tissue grinder for 1 min until it becomes minced for later use.
[0032] Weigh papain, add water to dissolve it, put it in a constant temperature oscillating water bath and heat it up to the set temperature, then add minced green squid meat, adjust the pH value with phosphate buffer (PBS, 0.5mol / L, pH=6.5), the enzyme untie.
[0033] The parameters of the enzymatic hydrolysis process are as follows: weigh 1000g of minced green fish meat, the amount of papain added is 2000-3000U / g, the enzymatic hydrolysis time is 3-5h, the pH is 6.5-7.0, and the temperature in the constant temperature oscillating water bath is 50± 2°C, after the hydrolysis is complete, take it out and place it in a boiling water bath to inactivate the enzyme for 10-15 minutes. Then centrifuge at 4000-5000r / min in a centrifuge for 10-15min, and tak...
Embodiment 1
[0038] The percentage by weight of each component of Portunus trituberculatus artificially compounded bait: 25% of fish meal, 55% of pregelatinized starch, 12% of Maillard reaction product of Qingzhan fish enzymatic hydrolyzate, and 8% of betaine.
Embodiment 2
[0040] The percentage by weight of each component of Portunus trituberculatus artificially compounded bait: 40% of fish meal, 40% of pregelatinized starch, 17% of Maillard reaction product of Qingzhan fish enzymatic hydrolyzate, and 3% of betaine.
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