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Method for manufacture of biofertilizer by pleuromutilin bacterial residue harmless treatment

A technology of harmless treatment and pleuromutilization, applied in the direction of organic fertilizers, etc., can solve the problems of increasing production cost, waste of resources, polluting the environment, etc., and achieves the goal of reducing the amount of ammonium sulfate, promoting growth and metabolism, and increasing the content of nitrogen sources. Effect

Inactive Publication Date: 2015-06-17
江苏赛奥生化有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the dry waste cells after extracting pleuromutilin in my country are about 15,000 tons. Incineration or deep burial will not only increase production costs, but also cause waste of resources and pollute the environment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0026] A biological fertilizer manufacturing method for the harmless treatment of pleuromutilin slag, comprising the following steps:

[0027] 1. Harmless treatment method of waste pleuromutilin residue

[0028] Treatment method: Put 100kg of dried mushroom residue with a protein content of 41.62% and a pleuromutilin residue of 0.236% into a 1000L enamel reaction tank, add 400L of ammonia water with a content of 1.7%, stir evenly, soak for 2 hours, and then steam Raise the temperature to 95°C for 1 h, and after natural cooling, adjust the pH to 9.0 with HCl for use. After testing, all the pleuromutilin in the treated bacteria residue and solution were inactivated, and no pleuromutilin was detected.

[0029] 2. Streptomyces flavinus biological fertilizer production technology

[0030] 1. Eggplant bottle slant seed culture

[0031] (1) Medium: 200g potato, 20g glucose, 20g agar.

[0032] Take 200g of potatoes, cut them into small pieces, add 400ml of water, boil them and...

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Abstract

The invention discloses a method for manufacture of a biofertilizer by pleuromutilin bacterial residue harmless treatment. The method comprises pleuromutilin bacterial residue harmless treatment and streptomycesmicroflavus biofertilizer preparation. The streptomycesmicroflavus biofertilizer preparation comprises eggplant-shaped bottle-based slant strain culture, shake flask-based liquid strain culture, 1500L strain tank-based culture and 15000L fermentation tank-based culture. The method removes pleuromutilin in residual bacterial residues, utilizes an organic nitrogen source and a growth factor as streptomycesmicroflavus fermentation mediums for biofertilizer production, and realizes recycle of waste pleuromutilin bacterial residues.

Description

technical field [0001] The invention relates to a biological bacterial fertilizer manufacturing method for harmless treatment of pleuromutilin bacterial residue. Background technique [0002] Pleuromutilin slag is the fermented waste mycelium after extracting pleuromutilin, which contains a large amount of bacterial protein, accounting for more than 40% of the dry weight, rich in vitamins, nucleic acids, growth factors and culture medium residues and other nutrients, and a small amount of pleuromutilin (about 0.3%) remains. In 2002, the Ministry of Agriculture, the Ministry of Health and other departments jointly issued a document prohibiting the use of untreated antibiotic residues as feed additives. According to regulations, antibiotic residues can only be burned or buried deep. At present, the dry waste cells after extracting pleuromutilin in my country are about 15,000 tons. Incineration or deep burial will not only increase production costs, but also cause waste of res...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05F11/08
Inventor 陈剑慧韩勤更姜剑国付慧萍林永彬鲁胜利周劲朱菊芬沈春梅杨锐
Owner 江苏赛奥生化有限公司