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Seedling breeding method using asparagus spores

A technology of asparagus and spores, which is applied in the field of marine algae reproduction, can solve problems such as germplasm degradation, degeneration, genetic variation, etc., and achieve the effects of alleviating germplasm degradation, solving provenance problems, and accelerating the speed.

Active Publication Date: 2015-03-11
OCEAN UNIV OF CHINA
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Problems solved by technology

[0002] At present, asparagus is cultivated by means of vegetative propagation through clipping seedlings of algae. In the long-term cultivation process, some problems are faced: one breeding seedling is single, and genetic variation and germplasm degradation are caused by the harsh environment of the sea area. The excellent traits of the parent cannot be maintained; 2. The problem of the source of improved seeds required for large-scale promotion
The seedlings required for cultivation are directly taken from the float method plus the harvested algae, accounting for about 20-30% of the total output, and reducing the benefits of cultivation; Due to the limitation and lack of sources, some people have proposed the strategy of indoor summer and cold storage preservation, but the indoor space and labor costs occupied by such a huge algal body are prohibitive; 4 Asparagus cultivation is mainly distributed in southern Fujian, Along the coast of Guangzhou, Rongcheng, Shandong and other places in the north
When the cultivation season comes, only the traditional methods of "south-to-north transfer" and "north-to-south transfer" can be used. Incomplete statistics transport costs account for 15% of the cost of seedlings, consuming a lot of manpower and material resources; Affected by the environment, seedling cultivation is unstable and controllability is poor
[0003] In the 1950s, my country began to carry out the artificial cultivation experiment of Gracilaria; in the early 1960s, the spore raising experiment of Gracilaria was carried out, but so far there has been no report on the indoor spore raising of Asparagus; in the 1970s, the research in my country was successful Semi-artificial Seedling Cultivation Technique of Gracilaria
It is impossible for a single improved species to be suitable for all cultivation sea areas, and there is a possibility of degradation

Method used

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  • Seedling breeding method using asparagus spores
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  • Seedling breeding method using asparagus spores

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A propagation method of asparagus, comprising the steps of:

[0039] 1) Spore collection

[0040] The collection box 6 is filled with seawater, the lower water layer is placed with a blank net curtain 4, and the mature asparagus 7 is placed on the upper water layer by horizontal clamping seedlings and is completely covered by seawater. The top of the collection box 6 is covered with a preservative film 5 Sealing, tracking and counting the spores on the net curtain 4 every 4 hours, under a microscope with a magnification of 10*10, the density of spores on each net curtain can reach 40-50 per field of view;

[0041] 2) Indoor cultivation

[0042] The net curtain with spores after step 1) is cultivated indoors for 1 week to one month, the temperature of cultivation is 20-25°C; the salinity is 30‰; the light intensity is 1000-4500lx, until the spores grow into discs or just begin Upright growth, the culture conditions in the previous week were 20°C, 1000lx, 30‰, 12 / 12h / h;...

Embodiment 2

[0048] The preparation method of net curtain 4 is:

[0049] 1) Make a rectangular net curtain frame 2, and fix a hanging ear 3 on each of the four corners of the net curtain frame 2;

[0050] 2) Wrap the cotton rope 1 on the net curtain frame 2 to make a net curtain 4;

[0051] 3) Apply the resin evenly to the front of the net curtain 4, and place it at 60°C for 6 hours or at room temperature (20-25°C) for 24 hours;

[0052] 4) After the resin on the front side of the net curtain 4 is solidified, apply resin on the reverse side according to step 3) and wait for it to solidify;

[0053]5) Soaking in seawater: Soak the net curtain 4 after step 4) in seawater for 4-6 days.

[0054] On the basis of the above scheme, the resin is prepared by mixing epoxy resin and polyamide resin in equal mass, adding 2 to 3 times the volume of industrial alcohol to dilute, and mixing evenly.

[0055] in:

[0056] Epoxy resin: produced by Zhonghua Group Jinan Resin Factory or Sino-foreign joint...

Embodiment 3

[0061] Selection of mature asparagus and daily release of spores:

[0062] Select mature tetraspores or fertilized and mature fruit sporophytes. When the algal body is mature, it can be judged according to its shape under the microscope, as follows figure 1 As shown, the mature fruit sporophyte parasitized on the female gametophyte and protruded from the surface of the algal body; as figure 2 As shown, the internal photo of the mature fruit sporophyte, the circle is the mature fruit sporophyte; as image 3 As shown, mature tetrasporocytes, the bright red surface is tetrasporangia.

[0063] The daily release law of spores: such as Figure 4 As shown, it took 7 days for mature fruit spores to disperse from the beginning to the end of dispersal. On the fourth day, a large amount of spores were released, and the amount of spores released was about 2000 spores per cystic fruit. Such as Figure 5 As shown, the release of tetrasporocytes lasts for about 10 days, begins to relea...

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Abstract

The invention relates to a seedling breeding method using asparagus spores. The method comprises the steps of (1) spore collection, (2) indoor cultivation and (3) sea area hanging cultivation and period control. The seedling breeding method using the asparagus spores has the advantages that provenances needed by large-area popularization can be obtained effectively; indoor storage of asparagus during summer is achieved; seedling breeding is high in stability and strong in controllability; cost and labor and material consumption caused by north-to-south transfer and south-to-north transfer are effectively reduced; compared with vegetative propagation, generative propagation of seedlings has the advantages of being capable of breeding a large number of seedlings with different genetic backgrounds, selectively cultivating seedlings adaptable to different sea areas, and easing idioplasm degeneration to a certain degree; improved variety screening during asparagus breeding work is accelerated.

Description

technical field [0001] The invention relates to a breeding method of marine algae, in particular to a breeding method of asparagus spore seedlings. Background technique [0002] At present, asparagus is cultivated by means of vegetative propagation through clipping seedlings of algae. In the long-term cultivation process, some problems are faced: one breeding seedling is single, and genetic variation and germplasm degradation are caused by the harsh environment of the sea area. The excellent traits of the parent cannot be maintained; 2. The problem of the source of improved seeds required for large-scale promotion. The seedlings required for cultivation are directly taken from the float method plus the harvested algae, accounting for about 20-30% of the total output, and reducing the benefits of cultivation; Due to the limitation and lack of sources, some people have proposed the strategy of indoor summer and cold storage preservation, but the indoor space and labor costs o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G33/00
Inventor 隋正红周伟王津果常连鹏丁弘叶胡依依
Owner OCEAN UNIV OF CHINA
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