Bergenia purpurascens(hook.f.et Thoms.)Engl. tender leaf tissue culture rapid propagation method

Abstract

The invention relates to a bergenia purpurascens(hook.f.et Thoms.)Engl. tender leaf tissue culture rapid propagation method. Under a natural wild condition, bergenia purpurascens(hook.f.et Thoms.)Engl. is low in emergence rate and has a low rhizome propagation coefficient, propagation by sowing seeds is low in emergence rate and has a low propagation coefficient, and cutting propagation can consume part of product organs, so that the production efficiency is reduced. The method comprises the following steps of: disinfecting and sterilizing an explant which serves as a raw material, performing induction culture to obtain callus, performing induction culture to obtain adventitious buds, performing proliferation culture on the buds, rooting culturing and hardening off seedlings and transplanting. The invention also provides formula of a culture medium used in the method. According to the method for performing tissue culture on bergenia purpurascens(hook.f.et Thoms.)Engl. tender leaves to generate seedlings, the seedlings of the bergenia purpurascens(hook.f.et Thoms.)Engl. tender leaves are more easily obtained when being compared with those from seeds and rhizomes, bergenia purpurascens(hook.f.et Thoms.)Engl. tender leaves are more easily obtained compared with seeds and rhizomes, and the propagation coefficient is high and not limited by the seasons and the climates; the demand for the seedlings on the production can be met at any time; furthermore, the tender leaf callus inductioncan be suitable for industrial production of various leaf medicinal components; moreover, the method is combined with an artificial mutation and transgenic technology, so that the germplasm can be improved, and a foundation is laid for the culture of new purple bergenia species.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of rock cabbage young leaves, belonging to the field of plant tissue culture, in particular to rapid propagation of plant organs. Background technique [0002] Rock cabbage [Bergenia purpurascens (Hook.f.et Thoms.) Engl.] is a perennial herb of Saxifragaceae (Saxifragaceae) Bergenia. value. Relying on a large amount of wild excavation alone can no longer meet its raw material supply and sustainable development needs, so the domestication and cultivation of rock cabbage has gradually attracted attention in recent years. At present, the seedling breeding of rock cabbage mostly adopts two methods of sowing seedlings and cutting seedlings. Rock cabbage seeds are invented seeds, without after-ripening dormancy, relatively small, thousand-grain weight is only 0.13g, and the emergence rate of natural sowing is low, only 22.5%, and the actual natural wild conditions are even lower, be...

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is how to overcome the defects that the low emergence rate of rock cabbage seeds and the low rhizome reproduction coefficient are not conducive to rapid and large-scale breeding of seedlings, and provide a method for tissue culture and rapid propagation of rock cabbage tender leaves

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