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Method for producing hyaluronic acid

A production method, hyaluronic acid technology, applied in the production field of hyaluronic acid, to achieve the effects of increased yield, low production cost, and high yield

Active Publication Date: 2013-11-20
ANYANG JIUZHOU PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] So far, there has been no report on the addition of sodium β-hydroxybutyrate and nicotinic acid to the medium for fermentative production of hyaluronic acid using Streptococcus zooepidemicus (HA-116ATCC39920)

Method used

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  • Method for producing hyaluronic acid

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Effect test

Embodiment 1

[0030] A production method of hyaluronic acid adopts Streptococcus zooepidemicus as the fermentation strain. Sodium β-hydroxybutyrate and nicotinic acid were added to the medium in the conventional method. The amount of sodium β-hydroxybutyrate added was 0.1% of the mass of the medium; the amount of nicotinic acid added was 0.05% of the mass of the medium; at the same time, the mass percentage of glucose in the medium was adjusted to 1.6%.

[0031] The obtained hyaluronic acid yield is 1.17g / L, the protein content in the hyaluronic acid is 0.025%, and the production cost is reduced by 21%.

Embodiment 2

[0033] A production method of hyaluronic acid adopts Streptococcus zooepidemicus as the fermentation strain. Sodium β-hydroxybutyrate and niacin were added to the medium. The amount of sodium β-hydroxybutyrate added was 1% of the mass of the medium; the amount of nicotinic acid added was 0.5% of the mass of the medium; at the same time, the mass percentage of glucose in the medium was adjusted to 0.8%.

[0034] The obtained hyaluronic acid yield is 1.2g / L, the protein content in the hyaluronic acid is 0.029%, and the production cost is reduced by 20%.

Embodiment 3

[0036] A production method of hyaluronic acid adopts Streptococcus zooepidemicus as the fermentation strain. Sodium β-hydroxybutyrate and niacin were added to the medium. The amount of sodium β-hydroxybutyrate added was 0.5% of the mass of the medium; the amount of nicotinic acid added was 0.2% of the mass of the medium; at the same time, the mass percentage of glucose in the medium was adjusted to 1.2%.

[0037] The yield of hyaluronic acid obtained is 1.22g / L, the protein content in the hyaluronic acid is 0.015%, and the production cost is reduced by 22%.

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Abstract

The invention particularly provides a method for producing hyaluronic acid. The method is characterized in that streptococcus zooepidemicus (HA-116ATCC39920) is adopted for fermentation and sodium beta-hydroxybutyrate and nicotinicacid are added into a culture medium. The method for producing hyaluronic acid provided by the invention has the advantages that the hyaluronic acid yield is high, the hyaluronic acid quality is good, and the production cost is low.

Description

technical field [0001] The invention particularly provides a method for producing hyaluronic acid with high yield. Background technique [0002] Hyaluronic acid is hyaluronic acid (Sodium Hyaluronate), hyaluronic acid, referred to as HA, is an acidic mucopolysaccharide. Its molecular structure is formed by repeatedly connecting N-acetylglucosamine and glucuronic acid through β-1,4 and β-1,3 glycosidic bonds, and its molecular weight is between 100,000 and 4,000,000. Two monosaccharides in the molecule, β-D-glucuronic acid (glucuronic acid) and N-acetylamino-D-glucosamine (N-acetyl glucosamine), are composed in a molar ratio of 1:1. [0003] Molecular formula: (C 14 h 20 NNaO 11 )n, CAS number: 9004-61-9, its disaccharide structure is as follows: [0004] [0005] Molecular structure of HA [0006] In 1934, Meyer, a professor of ophthalmology at Columbia University in the United States, first isolated the substance from the vitreous humor of bull eyes. In 1937, Kenda...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/26C12R1/46
Inventor 潘淑勇曹俊华魏淑丽
Owner ANYANG JIUZHOU PHARMA