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Novel broad-spectrum rice blast resistance allele pi21t and resistance application thereof

A rice blast disease and allele technology is applied to a new rice blast resistance new allele pi21t and its resistance application field, which can solve the problems of race specific resistance loss, loss of disease resistance, etc.

Inactive Publication Date: 2013-11-27
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

[0003] Rice blast is caused by rice blast fungus (Magnaporthe grisea Barr, the asexual generation is Pyricularia grisea Sacc) The races of Magnaporthe oryzae have a high degree of variability. With the continuous use of resistance genes, the genetic structure of the pathogen population will continue to change, and new physiological races will appear, resulting in the loss of race-specific resistance. Most resistant varieties gradually lose disease resistance after a few years of planting

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  • Novel broad-spectrum rice blast resistance allele pi21t and resistance application thereof
  • Novel broad-spectrum rice blast resistance allele pi21t and resistance application thereof
  • Novel broad-spectrum rice blast resistance allele pi21t and resistance application thereof

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Embodiment Construction

[0027] The methods used in the following examples are conventional methods unless otherwise specified.

[0028] (1) New alleles of broad-spectrum resistance to rice blast carried by wide-compatibility variety 02428 pi21t identification method

[0029] 1. Extraction of Genomic DNA of Wide Compatibility Variety 02428

[0030] The young leaves of wide-compatibility variety 02428 were taken, and rice genomic DNA was extracted by 2×CTAB method (2% CTAB, 1.4mol / L NaCl, 100mmol / L Tris-Cl, 20mmol / L EDTA, pH 8.0).

[0031] Put about 0.1g of rice leaf into a 2mL small centrifuge tube, freeze it in liquid nitrogen and smash it into powder with a bamboo stick; add 700μL of 2×CTAB extraction buffer solution preheated at 65°C, mix well and place at 65°C Water bath for 30 minutes, and mix once every 5 minutes; after the water bath, add 700 μL chloroform to each centrifuge tube, mix well by inverting up and down, extract for 5 minutes; 10000 rpm, centrifuge for 8 minutes, take 500 μL of s...

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Abstract

The invention belongs to the field of molecular genetics and relates to a novel broad-spectrum rice blast resistance allele pi21t and resistance application thereof. According to a cloned sequential design primer of the broad-spectrum rice blast resistance recessive allele pi21t, PCR amplification is performed on the wide compatibility variety 02428 which is used in high frequency in breeding, and sequencing comparative analysis is performed, so that the novel broad-spectrum rice blast resistance allele pi21t is identified in combination with a field rice blast inoculation identification result of the variety. The material has high resistance for popular microspecies of 6 rice blast groups in the Yangtze-Huaihe river basin, and provides new gene resources for breeding for rice blast resistance.

Description

technical field [0001] The present invention identifies a new allele for broad-spectrum rice blast resistance pi21t It belongs to the field of molecular genetics and is dedicated to the molecular design and breeding of rice blast resistance, in order to provide new resources for rice broad-spectrum blast resistance breeding, and to speed up rice broad-spectrum resistance to rice through molecular marker-assisted selection breeding. The process of blast breeding to improve the efficiency of disease resistance. Background technique [0002] Rice is one of the most important food crops in the world, and it is also the largest food crop in my country. Improving rice yield per unit area and total output plays a decisive role in ensuring my country's food security. Rice blast is the most important worldwide disease of rice, commonly known as rice "cancer", which spreads to more than 80 countries around the world, and occurs to varying degrees in my country every year. Severely di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415
Inventor 朱金燕杨杰仲维功王军范方军李文奇
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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