Application of aplysin in preparation of medicine for treating glioma

An Aplysin and glioma technology, applied in the field of biomedicine, can solve problems such as inability to effectively control the development of glioma, and achieve the effects of good market application prospects, less toxic and side effects, and good anti-tumor effects.

Inactive Publication Date: 2013-12-18
HOSPITAL ATTACHED TO QINGDAO UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

The current symptomatic treatment methods cannot effectively control the development of glioma. Therefore, finding natural and effective chemotherapy drugs to delay the progression of the disease and improve the survival rate has immeasurable clinical application value and broad economic and social benefits.
However, there is no research report on Aplysia in the treatment of glioma.

Method used

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  • Application of aplysin in preparation of medicine for treating glioma
  • Application of aplysin in preparation of medicine for treating glioma
  • Application of aplysin in preparation of medicine for treating glioma

Examples

Experimental program
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Embodiment 1

[0024] The experimental materials used in the present invention are as follows: C6 glioma cell line was purchased from Shanghai Institute of Cell Biology, Chinese Academy of Sciences; DMEM medium was purchased from GIBCO BRL Company of the United States; trypsin was purchased from Sigma Company of the United States; EGFR, COX-2, Bcl- 2. Bax, ERK, and PI3K monoclonal antibodies were purchased from Cell Signaling, USA; mouse anti-rat CD 8 Mcab (Serotec).

[0025] 1. Preparation of Aplysia

[0026] The present invention extracts Aplysia tristicha Tseng (Laurencia tristicha Tseng, Chang, E.Z.et.B.M.Xia) is red Rhodophyta, Rhodophyceae, Ceramiales, Cermiaceae, Laurencia. S. tricoleum was provided and identified by the Institute of Oceanology, Chinese Academy of Sciences.

[0027] The preparation method of Aplysia: the Aplysia three-row sample (5kg) air-dried at room temperature was soaked in ethanol with a volume fraction of 0.95 at room temperature for 3 days, extracted 3 times...

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Abstract

The invention discloses application of aplysin in preparation of a medicine for treating glioma, aiming at researching the anti-tumor activity of aplysin to glioma. The MTT (Methyl Thiazolyl Tetrazolium) experiment shows that the inhibition ratio of aplysin to C6 cell is increased along with the raise of the concentration of aplysin; after the concentration is raised beyond 80mcg/m, the inhibition ratio of C6 cell does not raise obviously, namely, the most effective anti-tumor concentration of aplysin is 80mc/ml; the best effect appears in 48 hours in light of the action time; with the prolonging of the time, the cell inhibition ratio does not raise obviously. The animal experiment result shows that after the dose of aplysin is increased, the life time of a rat is prolonged, the weight of glioma is reduced, and the infiltration of lymphocyte is improved; 40mg/kg.bw is the best dose for reaching the best effect. The experiment shows that aplysin belongs to natural marine drugs and has small toxic and side effects, so that aplysin can be used as the medicine for treating glioma; aplysin is good in market application prospect.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the use of aplysia in the preparation of medicine for treating glioma. Background technique [0002] Glioma is the most common intracranial malignant tumor, and has the highest intracranial incidence rate. The current treatment method is still mainly surgery, supplemented by radiotherapy and chemotherapy. However, surgery is difficult to remove completely, the recurrence rate and mortality rate are high, and the effects of radiotherapy and chemotherapy are not satisfactory. Although new treatment methods such as gene therapy and immunotherapy have also been applied, they have not achieved breakthroughs. The current symptomatic treatment methods cannot effectively control the development of glioma. Therefore, finding natural and effective chemotherapy drugs to delay the progression of the disease and improve the survival rate has immeasurable clinical application value and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/343A61P35/00
Inventor 宫安静梁惠窦梅贺娟
Owner HOSPITAL ATTACHED TO QINGDAO UNIV
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