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Detection method for bacteria attached to body surfaces of cyclops zooplankton

A zooplankton and detection method technology, applied in the field of microorganisms, can solve the problems of low detection value and large fluctuation, and achieve the effects of high accuracy, reduced desorption time, and stable and reliable detection results.

Inactive Publication Date: 2014-12-03
HOHAI UNIV
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AI Technical Summary

Problems solved by technology

After using this method to desorb the bacteria attached to the surface of Cyclops zooplankton, the medium culture test is carried out, and there are disadvantages such as low detection value and large fluctuation of the bacteria attached to the surface of Cyclops zooplankton.

Method used

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  • Detection method for bacteria attached to body surfaces of cyclops zooplankton

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Experimental program
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Embodiment 1

[0017] The hydrozoan zooplankton used in the experiment was taken from the raw water of Taihu Lake. The genus Limnoithona sinensis was taken from the raw water of Taihu Lake. The hydrozoan and the raw water were sampled and brought back to the laboratory for future use.

[0018] Step 1, use a 3mL Pasteur pipette to suck up the adult Cyclops in the water sample one by one, and place them on a 30 μm sterile filter; 15 adult Cyclops are taken as a group.

[0019] Step 2. Under aseptic conditions, rinse the Cyclops on the sterile filter with sterile ultrapure water. The amount of sterile water for each rinse is 25mL. After the last two rinses, the rinse water flowing out of the filter must not The detection of bacteria (the method for the determination of the total number of bacteria in the standard test method for drinking water (microbiological indicators) (GB / T5750.12-2006)) is the basis for the end of flushing.

[0020] It has been proved by experiments that flushing the Cyclo...

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Abstract

The invention provides a detection method for bacteria attached to the body surface of cyclops zooplankton. The method comprises the following steps: 1. taking cyclops in a water sample, and washing the cyclops till no bacterium is detected; 2. placing the cyclops obtained in the step 1 into a centrifuge tube, and adding a desorption reagent; 3. placing the centrifuge tube in the step 2 into a centrifugal machine for centrifugal treatment; 4. taking supernate after centrifugation, carrying out bacterial culture on the supernate by a testing method of total number of bacteria in household drinking water standard test method, and counting cultivated bacterial colonies. According to the method, the operating steps are simple, the detection period is short, and the remarkable detection effect to bacteria attached to the body surface of cyclops zooplankton is remarkable.

Description

technical field [0001] The invention belongs to the field of microbes, and in particular relates to a method for detecting bacteria attached to the body surface of hydrozoan zooplankton. Background technique [0002] In recent years, there have been frequent incidents of hydrozoan zooplankton penetrating the drinking water treatment process system and entering the pipe network in China, which has threatened and challenged the safety of drinking water. The practice of the water plant has proved that the water treatment process cannot completely remove the water flea, and the adult Xenophobia is visible to the naked eye, and its appearance in the water treatment process system brings about sensory problems for users; at the same time, the Xenophobia can be used as a carrier of bacteria Or other intermediate hosts of pathogenic protozoa, and provide shelter to them, causing water quality biosafety problems. Studies have shown that bacteria in some water can stably attach to th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/06
Inventor 林涛陈卫蔡博吴寿可
Owner HOHAI UNIV
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