Method for extracting L-DOPA from velvet beans

A technology of levodopa and cat beans is applied in the field of preparation of plant extracts, which can solve the problems of excessive heavy metals in products, influence product quality, and many steps, and achieve the effects of simple method, saving extraction reagents, and few steps.

Active Publication Date: 2013-12-25
三原金瑞生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has many steps and takes a long time. A large amount of inorganic salts and other substances are added in the preparation process, which may easily cause heavy metals in the product to exceed the standard, and affect the quality of the product.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Extract levodopa from cat beans, the steps are as follows:

[0023] 1) Subcritical extraction is carried out with hydrochloric acid after the cat beans are crushed. The mass fraction of hydrochloric acid is 2‰, the injection volume is 1.5 times the weight of cat beans, the extraction pressure is 5MPa, the temperature is 80°C, and the time is 1h. Concentrate to twice the weight of cat beans, let stand at room temperature overnight, and crystallize to obtain a crude extract; the yield of the crude extract is 4.1%, and the weight content of levodopa is 80%;

[0024] 2) Dissolve the crude extract with 1mol / L hydrochloric acid, add activated carbon with a weight of 1‰ after concentration of the extract, reflux for 30min, filter to remove the activated carbon, adjust the pH of the filtrate to 3.5 with ammonia water, stand at room temperature overnight, and crystallize , to obtain a finished product after drying; the product yield of the finished product is 3.0%, and the weigh...

Embodiment 2

[0026] Extract levodopa from cat beans, the steps are as follows:

[0027] 1) Subcritical extraction is carried out with hydrochloric acid after the cat beans are crushed. The mass fraction of hydrochloric acid is 1‰, the injection volume is 1.5 times the weight of cat beans, the extraction pressure is 5.5MPa, the temperature is 75°C, and the time is 1h. The liquid is concentrated to 3 times of the cat bean weight, left standing at room temperature, overnight, and crystallized to obtain a crude extract; the yield of the crude extract is 3.9%, and the weight content of levodopa is 78%;

[0028] 2) Dissolve the crude extract with 0.5mol / L hydrochloric acid, add activated carbon with a weight of 1.5‰ after concentration of the extract, reflux for 60min, filter to remove the activated carbon, adjust the pH of the filtrate to 3.0 with ammonia water, let it stand at room temperature overnight, and analyze crystal, and the finished product is obtained after drying; the product yield ...

Embodiment 3

[0030] Extract levodopa from cat beans, the steps are as follows:

[0031] 1) Subcritical extraction is carried out with hydrochloric acid after crushing cat beans. The mass fraction of hydrochloric acid is 2‰, the injection volume is twice the weight of cat beans, the extraction pressure is 4.5MPa, the temperature is 80°C, and the time is 1.5h. The extract is concentrated to 1 times the weight of cat beans, left standing at room temperature overnight, and crystallized to obtain a crude extract; the yield of the crude extract is 3.8%, and the weight content of levodopa is 79%;

[0032] 2) Dissolve the crude extract with 1mol / L hydrochloric acid, add activated carbon with a weight of 2‰ after concentration of the extract, reflux for 20 minutes, filter to remove the activated carbon, adjust the pH of the filtrate to 3.5 with ammonia water, stand at room temperature overnight, and crystallize , to obtain a finished product after drying; the product yield of the finished product i...

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PUM

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Abstract

The invention discloses a method for extracting L-DOPA from velvet beans. The method includes the steps that after the velvet beans are crushed, hydrochloric acid with the mass ratio being 1%.-3%. is used for conducting subcritical extraction, after an extracting solution is concentrated, standing, staying overnight and devitrification are carried out, and accordingly a crude extract is obtained; after the crude extract is dissolved by diluted hydrochloric acid, activated carbon is added for conducting reflux, then the activated carbon is filtered and removed, the pH of the filter liquor is adjusted to be 3.0-4.0 by using an alkaline agent, standing, staying overnight and devitrification are carried out, and after drying is completed, a finished product can be obtained. The method for extracting the L-DOPA from the velvet beans is simple in method, few in step, short in time, and a reagent is saved; the yield of the finished product is 2.8%-3.0%, the content can reach up to 98%, a great number of substances such as inorganic salt do not need to be added in the extracting process, exceeding of heavy metal and the like is avoided, and therefore product quality can not be affected.

Description

technical field [0001] The invention relates to a preparation method of a plant extract, in particular to a method for extracting levodopa from cat beans. Background technique [0002] Levodopa (L-DOPA, full name 3,4-dihydroxyphenylalanine) is an anti-parkinsonian drug, which enters the brain tissue through the blood-brain barrier to play a role, and is suitable for primary parkinsonia and non-pharmaceutical tremor paralytic syndrome. The levodopa content of cat beans is as high as 5.22% to 9.94%. At present, there is a certain history of extracting natural levodopa from cat beans. The traditional extraction process mostly uses the acid-water-alcohol method, and the yield of levodopa is only about 1.5%. The biggest disadvantage of this process is that a large amount of extractant needs to be evaporated, which requires high energy consumption, low yield, large investment in equipment, and high cost. [0003] Wei Guofeng, Huang Zuliang, He Youcheng, etc. compared the effect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C229/36C07C227/40
Inventor 蒋元勇文继承朱昂辉易华李丽金雍小军
Owner 三原金瑞生物工程有限公司
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