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Extraction method and application of piperine

An extraction method, piperine technology, applied in the field of traditional Chinese medicine extraction, can solve problems such as rough process, affecting clinical application, and many impurities

Active Publication Date: 2014-01-01
陕西华泰药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the prior art, there is no report on the joint application of piperine in the extraction and preparation of supercritical and microwave technology. However, the current extraction method is rough and backward in technology and has many impurities, which leads to excessive dosage for patients, which is inconvenient to take and seriously affects This product has been applied clinically

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take 1000g of pepper and add it to CO 2 In the supercritical extractor, ethanol is used as the entrainer, the volume percentage of the entrainer in the total extraction solvent is 4%, the extraction pressure is 15MPa, the temperature is 30°C, and CO 2 The flow rate is 1m1 / g crude drug·min, the extraction time is 150min, and the supercritical extract is obtained. Then 70% ethanol is added, and the microwave extraction is carried out in the microwave extraction device. The extraction power is 400W, the extraction is 2 times, each time is 4 minutes, each time added The amount of 70% ethanol is 2000g, the extract is obtained, concentrated, and added to the D101 macroporous adsorption resin column, eluted with 50% ethanol, collected 5 times the column volume of the eluate, recovered the ethanol under reduced pressure, concentrated and dried to obtain pepper Alkaline extract, the content of piperine determined by high performance liquid chromatography is 98.4%.

Embodiment 2

[0020] Take 1000g of pepper and add it to CO 2 In the supercritical extractor, ethanol is used as the entrainer, and the volume percentage of the entrainer in the total extraction solvent is 6%, the extraction pressure is 30MPa, the temperature is 50°C, and CO 2 The flow rate is 3m1 / g crude drug·min, the extraction time is 180min, and the supercritical extract is obtained, then 70% ethanol is added, and the microwave extraction is carried out in the microwave extraction device. The extraction power is 600W, the extraction is 2 times, each time is 8 minutes, and each time is added The amount of 70% ethanol is 2000g, the extract is obtained, concentrated, and added to the D101 macroporous adsorption resin column, eluted with 50% ethanol, collected 5 times the column volume of the eluate, recovered the ethanol under reduced pressure, concentrated and dried to obtain pepper Alkaline extract, the content of piperine determined by high performance liquid chromatography is 98.5%.

Embodiment 3

[0022] Take 1000g of pepper and add it to CO 2 In the supercritical extractor, ethanol is used as the entrainer. The entrainer accounts for 5% of the total extraction solvent. The extraction pressure is 20MPa, the temperature is 40°C, and CO 2 The flow rate is 2m1 / g crude drug·min, the extraction time is 160min, and the supercritical extract is obtained. Then 70% ethanol is added, and the microwave extraction is carried out in the microwave extraction device. The extraction power is 500W, the extraction is 2 times, each time is 6 minutes, and each time is added The amount of 70% ethanol is 2000g, the extract is obtained, concentrated, and added to the D101 macroporous adsorption resin column, eluted with 50% ethanol, collected 5 times the column volume of the eluate, recovered the ethanol under reduced pressure, concentrated and dried to obtain pepper Alkaline extract, the content of piperine determined by high performance liquid chromatography was 99.1%.

[0023] In the above-men...

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Abstract

The invention provides an extraction method of piperine, which is obtained by supercritical extraction and microwave extraction. Thus, the content is greatly improved. The invention also provides application of piperine in preparation of drugs suppressing proliferation of human cervical carcinoma cell HELA cells and in preparation of drugs increasing bone mineral density.

Description

Technical field [0001] The invention relates to the technical field of traditional Chinese medicine extraction, in particular to an extraction method and application of piperine. Background technique [0002] Piperine is a broad-spectrum anticonvulsant. It has a good antagonistic effect on experimental electroconvulsions in mice. It has good resistance to pentylenetetrazol, picrotoxin, strychnine, as well as intraventricular injection tube curaline, glutamate, etc. The convulsive seizures and auditory seizures caused have different degrees of antagonism. It is also effective for certain types of epilepsy. Piperine is more toxic to flies than pyrethrum. [0003] In the prior art, there has not been a report on the combined application of piperine in the extraction and preparation of supercritical and microwave technology. However, the current extraction method is rough, backward, and contains many impurities, which leads to excessive consumption of patients, inconvenience to take,...

Claims

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Application Information

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IPC IPC(8): C07D317/60A61P35/00A61P19/10
CPCA61K31/4525C07D317/60Y02P20/54
Inventor 不公告发明人
Owner 陕西华泰药业有限公司
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