Operation pipe system for cell factory and application thereof

Abstract

The invention provides an operation pipe system for a cell factory. The operation pipe system comprises a first cell factory, a liquid inlet pipe and a liquid outlet pipe, wherein the liquid inlet pipe and the liquid outlet pipe are connected to the cell factory; the liquid inlet pipe and the liquid outlet pipe can be alternatively connected to a washing digestion system, a poison receiving system or a harvesting system. The invention also discloses a method for carrying out washing digestion, poison receiving or harvesting of the cell factory by using the system. By adopting the operation pipe system, the defects of long operation time, fussy operation, large batch, easy pollution, uneven cell digestion degree and the like when large-scale cell and virus cultivation is carried out by using the cell factory at present are overcome; a new pipe connection system for an operation of the cell factory is provided. The operation pipe system is respectively applicable to cell passage, poison receiving and harvesting operations in large-scale cell cultivation. By adopting the method, the service efficiency of an operation space can be increased; the operation time is shortened; the operation is relatively simple; the risk of pollution is reduced. Thus, the enlarge cultivation efficiency of the cells is improved.

Description

technical field [0001] The invention belongs to the field of biochemistry, and in particular relates to a virus suspension preparation system and its application. Background technique [0002] The cell factory is a commonly used cell culture tool in the biological and pharmaceutical industries, and is mainly suitable for industrialized mass production, such as vaccines, monoclonal antibodies or the pharmaceutical industry. It is not only suitable for adherent cell culture but also for suspension cell culture. Its culture mode is the same as small-scale cell bottle culture, so it can be scaled up. Commonly used cell factory scales are 2-layer, 4-layer, 10-layer and 40-layer. [0003] The commonly used cell factories in the prior art operate with a small amount of liquid and are relatively simple to operate. Usually, a simple pipeline system is used to pass the liquid in and out through the small opening of the cell factory by means of gravity difference. If this method is u...

AI Technical Summary

Problems solved by technology

If this method is used in a 40-layer cell factory, the connecting pipes need to be replaced many times. Not only is the operation speed slow, errors are prone to occur when multiple cell factories are operated at the same time, and the inlet and outlet parts of the cell factory are exposed to the air. Longer time, increased risk of contamination

When performing large-scale cell and virus culture, the shortcomings such as long operation time, cumbersome operation, large batch size, easy contamination, and uneven cell digestion are particularly obvious.

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