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Method for culturing anchorage dependent animal cell to produce virus by using magnetic stable fluidized bed

A technology for producing animal cells and viruses, applied in the direction of supporting/immobilizing microorganisms, culturing devices for tissue cells/viruses, and methods for stress-stimulating microbial growth, etc., to reduce damage, increase packing density, and improve the ability to produce viruses Effect

Inactive Publication Date: 2003-09-17
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there has been no report on the use of a magnetically stable fluidized bed to cultivate animal cells to produce viruses.

Method used

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  • Method for culturing anchorage dependent animal cell to produce virus by using magnetic stable fluidized bed
  • Method for culturing anchorage dependent animal cell to produce virus by using magnetic stable fluidized bed
  • Method for culturing anchorage dependent animal cell to produce virus by using magnetic stable fluidized bed

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Experimental program
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Embodiment Construction

[0030] figure 1 It is drawn according to the control elements and sensors are all installed on the oxygenation device, and its control elements and sensors can also be installed on the main body of the magnetically stable fluidized bed; the window, inlet hole and non-production necessary but in the equipment are not shown in the figure Valves, pipelines, inlets and outlets, etc. required for maintenance, overhaul or cleaning; figure 1 Other equipment, sensors and sampling devices for analysis, detection and control are not shown in the figure, such as tail gas analysis, culture medium composition analysis, etc.; mechanical defoaming device or defoamer can be used in the oxygenation device, which are not shown; components , The connection method of the equipment is not shown, such as flanges, bolts, nuts, throat hoops, welding points, etc. The material, model and specification are not marked; figure 1 The control device in the figure is only a schematic diagram, and products ...

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PUM

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Abstract

The method for producing virus by culturing anchorage-dependent animal cell through magnetic-stable fluidized bed includes the following steps: cleaning and sterilizing culture system and pretreated microcarrier, injecting cell culture medium and soaking microcarrier for a proper time, inoculating and making cell anchor on microcarrier, making culture medium circulate, charging oxygen, removing part of culture medium and at the same time supplementing fresh culture medium and implement cell culture process, changing virus culture medium, inoculating virogeny, making virus completely be adsorbed on the cell, making culture medium circulate and timely changing liquid discharged from culture medium in magnetic stable fluidized bed to liquid product outlet to storage tank so as to continuously or batchwise collect virus. Said invention can be used for culturing Vero cell to produce encephalitis B virus, cell density can be up to above 10 to the power 8 cells / ml, and virus titer is up to 8.5 TCID.

Description

technical field [0001] The invention belongs to animal cell culture technology, in particular to a method for cultivating anchorage-dependent animal cells and producing viruses with a magnetically stable fluidized bed. Background technique [0002] Large-scale cultivation of animal cells has become more and more important in modern biotechnology and pharmaceutical industries, and has been used to produce viruses such as hepatitis B, Japanese encephalitis, and rabies to prepare vaccines. [0003] Magnetically stabilized fluidized bed (Magnetically stabilized fluidized bed) is to add a magnetic field to the ordinary fluidized bed, and the particles in the fluidized bed are also magnetic. The particles form chains in the magnetic field, and when the liquid passes through the fluidized bed, the particle layer expands evenly, and the liquid is a piston flow. The magnetically stable fluidized bed not only has the pressure drop and fluidization charac...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12N5/16C12N7/00
CPCC12M25/20C12M35/06
Inventor 丛威吕秀菊高红亮欧阳藩
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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