Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kelp gametophyte lhcf 6 gene promoter and application thereof

A technology of genes and recombinant vectors, applied in the field of genetic engineering, can solve problems that have not been reported

Inactive Publication Date: 2014-01-08
SHANGHAI OCEAN UNIV
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the sequence of the lhcf6 gene promoter in the gametophyte of S. japonica.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kelp gametophyte lhcf 6 gene promoter and application thereof
  • Kelp gametophyte lhcf 6 gene promoter and application thereof
  • Kelp gametophyte lhcf 6 gene promoter and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Materials

[0038] 1) Kelp ( S. japonica ) female and male gametophytes were taken from our laboratory. At a temperature of 17°C and a light intensity of 40 μmol·m -2 ·s -1 , light / dark ratio of 16h / 8h under the conditions of cultivation, the medium is PES, the specific formula see literature: Starr RC, Zeikus JA (1993) UTEX-The culture collection of algae at the University of Texas at Austin. J Phycol 29 (Suppl): 1-106.

[0039] 2) Chlamydomonas reinhardtii ( C. reinhardtii ) is the cell wall deletion mutant strain CC-3491, which was purchased from the Chlamydomonas Center of Duke University, USA. At a temperature of 25°C and a light intensity of 70 μmol·m -2 ·s -1, Light / dark ratio is cultivated under the condition of 12h / 12h, and culture medium is TAP, and specific formula sees literature: Harris, E.H. (1989): The Chlamydomonas sourcebook: a comprehensive guide to biology and laboratory use. Academic Press, San Diego, 780pp.

[0040] 2. Method

[0041...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a kelp gametophyte lhcf 6 gene promoter and application thereof, specifically to the sequence of the kelp gametophyte lhcf 6 gene promoter, application of the promoter sequence in promotion of expression of a target gene by algae, a recombinant vector containing the promoter sequence and a preparation method for transgenic algae based on the promoter sequence. According to the invention, the kelp gametophyte lhcf 6 gene promoter obtained through cloning is connected with a fusion expression vector, and an electroporation process is adopted for conversion of Chlamydomonas reinhardtii; it is proved that the promoter has almost similar efficiency in driving expression of a target gene compared with a (CaMV)35S promoter, a basis is laid for further analysis of differential expression mechanisms of the lhcf gene in female gametophyte and male gametophyte, conditions are provided for artificial regulation and control of expression of related genes of algae, and the promoter is originated from kelp, having good safety.

Description

technical field [0001] The present invention relates to the technical field of genetic engineering, in particular, relates to kelp gametophyte lhcf 6 Gene promoters and their applications. Background technique [0002] Light-harvesting protein complex (LHC) is a type of protein embedded in the thylakoid membrane of algae and higher plants to bind antennae or auxiliary pigments and capture and transmit light energy for the photosynthetic reaction center. Under the appropriate light intensity, the LHC of algae plays the role of binding pigments and transfers the light energy captured by the pigments to the photoreaction center for photosynthesis; when the light intensity exceeds the maximum luminous flux required for photosynthesis, the algal cells adjust The expression of LHC gene can reduce the pigment bound on the thylakoid membrane and reduce the transmission of light energy to protect chloroplasts from irreparable damage caused by light inhibition; but under stress condi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/113C12N15/79A01H13/00C12R1/89
Inventor 毕燕会宁璞周志刚
Owner SHANGHAI OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com