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Method for detecting insecticide resistance of green peach aphids

A technology for insecticide resistance and green peach aphid, which is applied in the field of monitoring and measuring the resistance of agricultural pests. It can solve the problems of large number of insects and complicated operation procedures, and achieve the effect of less insects, simple operation, fast and accurate identification

Active Publication Date: 2015-06-03
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The prior art uses biochemical methods to measure the drug resistance of peach aphids. Generally, a spectrophotometer is used to measure the specific activity of the population. This method not only uses a large number of insects, but also has complicated operating procedures.

Method used

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  • Method for detecting insecticide resistance of green peach aphids
  • Method for detecting insecticide resistance of green peach aphids
  • Method for detecting insecticide resistance of green peach aphids

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Obtaining of embodiment 1 diagnostic dosage

[0033] 1. Research object

[0034] The research object of this patent is the sensitive population of green peach aphids raised in the laboratory and back-selected for multiple generations.

[0035] 2. Method

[0036] For the test drugs pirimicarb and omethoate, the inhibition curve of pirimicarb and omethoate and the corresponding diagnostic dose (IC 90 ).

[0037] 2.1 Collection and processing of samples of peach aphid for testing

[0038] 120 apterous adult aphids of indoor sensitive strains were selected and placed in 1.5ml centrifuge tubes, which were quickly frozen in liquid nitrogen and used for the preparation of enzyme solution.

[0039] 2.2 Preparation of reaction reagents

[0040] pH 7.5, 0.1M phosphate buffer, 2.5mM substrate ATChI, 37.5μM chromogenic reagent DTNB, wherein the substrate and chromogenic reagent need to be stored in a refrigerator at 4°C.

[0041] 2.3 Preparation of enzyme solution

[0042] U...

Embodiment 2

[0050] Example 2 Diagnostic Dosage Detection of Peach Aphid Resistance

[0051] Specific operation:

[0052] 1. Collection and processing of samples of peach aphid for testing

[0053] Select single-headed wingless adult aphids of the population to be tested in a 1.5ml centrifuge tube, freeze them in liquid nitrogen and use them for the preparation of enzyme solution, and measure at least 100 aphids for each population to be tested.

[0054] 2. Preparation of Reagents

[0055] pH 7.5, 0.1M phosphate buffer, 2.5mM substrate ATChI, 37.5μM chromogenic reagent DTNB, wherein the substrate and chromogenic reagent need to be stored in a refrigerator at 4°C.

[0056]3. Preparation of Enzyme Solution

[0057] Use the peach aphid sample in 1, add 200 μl of phosphate buffer, homogenize in ice bath (the homogenizer and buffer need to be pre-cooled for 5 minutes), enzyme solution at 10800g, and centrifuge at 4°C for 15 minutes.

[0058] 4. Kinetic determination of microplate reader

...

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Abstract

The invention provides a method for detecting insecticide resistance of green peach aphids. The method includes under a diagnostic dose, adopting a microplate reader kinetic method to detect resistance of field population individuals of the green peach aphids to organophosphorus or carbamate insecticide, wherein the organophosphorus insecticide is omethoate whose diagnostic dose is 180.43 muM, and the carbamate insecticide is pirimicarb whose diagnostic dose is 18.85 muM.

Description

technical field [0001] The invention relates to a method for measuring the pesticide resistance of agricultural pests, specifically, a method for detecting the pesticide resistance of peach aphids to two series of insecticides, organophosphorus and carbamate, with a diagnostic dose. Background technique [0002] Green peach aphid is one of the most widely distributed and important pests in the world. It can feed on more than 400 kinds of plants in more than 50 families. It can not only directly suck the nutrients of plants, cause serious dehydration and malnutrition of plants, but also spread viruses. , cause greater indirect damage to plants, and cause huge economic losses to agricultural production. For a long time, the control of green peach aphid has been mainly based on chemical control. Due to the large, continuous and irrational use of chemical insecticides, peach aphids in many countries and regions have developed varying degrees of resistance to commonly used insect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53
CPCG01N33/5085G01N2333/43504G01N2430/10
Inventor 高希武刘晓岚汤秋玲
Owner CHINA AGRI UNIV
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