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Golgi protein gp73 quantitative assay kit and its detection method

A Golgi body and detection method technology, applied in biological testing, measuring devices, material inspection products, etc., can solve problems such as weak specificity, low sensitivity, and complicated operation, and achieve short-term, simple operation, high sensitivity and specificity sexual effect

Inactive Publication Date: 2015-10-14
JIANGSU FLON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In previous studies, Western blot method was mostly used. This method is not only low in sensitivity and specificity, but also complicated in operation and cannot be widely used in clinical practice.
According to the patent search, there are many detection methods for GP73, including ELISA, genetic diagnosis, etc., but there is no application of plate-type magnetic particle chemiluminescence immunoassay technology to the detection of Golgi protein GP73.

Method used

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  • Golgi protein gp73 quantitative assay kit and its detection method
  • Golgi protein gp73 quantitative assay kit and its detection method
  • Golgi protein gp73 quantitative assay kit and its detection method

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Embodiment Construction

[0074] see Figure 1-Figure 3 , the Golgi protein GP73 quantitative assay kit of the present invention comprises a box body 1 and a box cover 2 above the box body, and the box body 1 is provided with a microporous plate 6 and a foamed plastic container at the bottom of the box body 1 Supporting plate 3, wherein microporous plate 6 is made up of 48 or 96 micropores 7, has a plurality of holes 4 on the supporting plate 3, is placed altogether in described hole 4 eight reagent bottles 5, and described eight reagent bottles 5 contains magnetic separation reagents, enzyme reactants, stability enhancers, calibrators, quality control products, cleaning concentrates, diluents and substrate solutions, in which the magnetic separation reagents contain magnetic particles.

[0075] Preparation

[0076] Step 1, Preparation of Magnetic Separation Reagent

[0077] 1. Preparation of magnetic bead buffer

[0078] 1. Weigh 4.58g of TRIS and 6.81g of NaCl into a 1L container, then weigh 0.96...

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Abstract

The invention relates to a quantitative determination kit for a Golgi protease GP73 and a detection method of thereof. The quantitative determination kit comprises the following seven steps of preparing a magnetic separation reagent; preparing an enzyme preparing reactant; preparing an enhancer; preparing a standardizing product dilution; preparing a standardizing product and a quality control product; preparing a cleaning concentrated solution; preparing a substrate solution. The quantitative determination kit and the detection method have the advantages that the sensitivity and the specificity are high, the time for acquiring a detection result is short, the operation manner is simple and convenient, and the significance on the early diagnosis of a liver cancer is important.

Description

technical field [0001] The invention relates to a kit for quantitative determination of Golgi protein GP73 and a detection method thereof, which are used for quantitative determination of the content of Golgi protein GP73 in sample serum in vitro. Background technique [0002] The incidence of liver cancer ranks fourth in the world and the second in China, and it is increasing year by year worldwide, with a total incidence of more than 560,000. Liver cancer is relatively hidden at the beginning of its onset, and it is difficult to detect it clinically. The detection of clinical diagnosis is generally late, and the treatment effect is poor. Early detection of liver cancer and timely treatment can improve the 5-year survival rate of patients. Therefore, early detection of liver cancer has important clinical significance significance. [0003] Currently, liver cancer detection methods include liver ultrasound image analysis and serological marker detection, among which the mos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/531
CPCG01N33/54326G01N33/54366G01N33/54393G01N33/57438
Inventor 奚伟红王布强王京严子禾高淑舫高磊
Owner JIANGSU FLON BIOTECH