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A method for extracting flavonoids in tea seeds by aqueous enzymatic method-semi-bionic method

A semi-bionic method and tea seed technology, which is applied in the field of extracting flavonoids from tea seeds by combining the aqueous enzymatic method and semi-bionic method, can solve the problems of limited extraction rate, insufficient development and utilization of tea seeds, and less research and development of flavonoids.

Inactive Publication Date: 2016-11-30
HUAQIAO UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] my country is a big tea-producing country with the largest planting area and output in the world. It is rich in tea tree resources and has an annual output of more than 800 million kilograms of tea seeds. However, people have underdeveloped and utilized tea seeds for a long time, and most of the tea seeds have been wasted. Now, the research on tea seeds at home and abroad is mainly focused on the extraction and refining of tea seed oil and the extraction of tea saponin, while the research and development of flavonoids is less. It is of great significance to develop tea seed flavonoids and avoid waste of resources. and broad prospects
[0003] The extraction of flavonoids by aqueous enzymatic method is a relatively efficient and environmentally friendly method, but the extraction rate of the aqueous enzymatic method is not ideal, which causes waste of flavonoid resources to a certain extent, and due to the presence of amphoteric macromolecular substances such as proteins in tea seeds, The o / w emulsion (emulsion) is inevitably formed in the oil / water-rich aqueous enzymatic flavonoid extraction system, which not only brings great challenges to the separation of the system, but also severely limits the extraction rate

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  • A method for extracting flavonoids in tea seeds by aqueous enzymatic method-semi-bionic method
  • A method for extracting flavonoids in tea seeds by aqueous enzymatic method-semi-bionic method
  • A method for extracting flavonoids in tea seeds by aqueous enzymatic method-semi-bionic method

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Embodiment 1

[0058] The method for extracting flavonoids in tea seeds by aqueous enzymatic method-semi-bionic method comprises the following steps:

[0059] (1) Preparation of tea seed powder: shell the tea seeds, crush and sieve, dry to constant weight to obtain tea seed powder;

[0060] (2) Aqueous enzymatic extraction: According to the weight / volume ratio of tea seed powder and reaction liquid as 1:4, weigh 10g of tea seed powder, add some water first, and heat for 4 minutes to eliminate the enzyme activity of tea seed powder itself , then cooled to room temperature, adding the prepared enzyme solution and the remaining water to obtain a mixed solution of tea seed powder, stirred evenly, and enzymatically hydrolyzed at 53°C for 0.85h under stirring conditions; the enzyme solution was 100mg of cellulase dissolved Quickly prepared in 10ml of distilled water at 0-5°C;

[0061] (3) Semi-bionic extraction: add ethanol and water to the mixed solution of tea seed powder after enzymolysis, so ...

Embodiment 2

[0063] The method for extracting flavonoids in tea seeds by aqueous enzymatic method-semi-bionic method comprises the following steps:

[0064] (1) Preparation of tea seed powder: shell the tea seeds, crush and sieve, dry to constant weight to obtain tea seed powder;

[0065] (2) Aqueous enzymatic extraction: According to the weight / volume ratio of tea seed powder and reaction solution as 1:3.5, weigh 10g of tea seed powder, add some water first, and heat for 3 minutes to eliminate the enzyme activity of tea seed powder itself , then cooled to room temperature, added the prepared enzyme solution and the remaining water to obtain the tea seed powder mixture, stirred evenly, and enzymatically hydrolyzed at 51°C for 2.0h under stirring conditions; the enzyme solution was cellulase dissolved in Quickly prepared in partially distilled water at 0-5°C, the dosage of cellulase is 7mg / g tea seed powder;

[0066] (3) Semi-bionic extraction: add ethanol and water to the mixed solution o...

Embodiment 3

[0068] The method for extracting flavonoids in tea seeds by aqueous enzymatic method-semi-bionic method comprises the following steps:

[0069] (1) Preparation of tea seed powder: shell the tea seeds, crush and sieve, dry to constant weight to obtain tea seed powder;

[0070] (2) Aqueous enzymatic extraction: according to the material-liquid weight / volume ratio of tea seed powder and reaction solution as 1:5.5, weigh 10g of tea seed powder, add some water first, and heat for 8 minutes to eliminate the enzyme activity of tea seed powder itself , then cooled to room temperature, adding the prepared enzyme solution and the remaining water to obtain the tea seed powder mixture, stirred evenly, and enzymatically hydrolyzed at 55°C for 0.5h under stirring conditions; the enzyme solution was cellulase dissolved in Quickly prepared in partially distilled water at 0-5°C, the dosage of cellulase is 11mg / g tea seed powder;

[0071] (3) Semi-bionic extraction: add water and ethanol to th...

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Abstract

The invention discloses a method for extracting flavonoids from tea seeds by hydroenzyme-semi-bionic method, which comprises the following steps: (1) drying tea seeds, shelling, crushing and sieving; (2) weighing tea seed powder, adding part water, heat to inactivate the enzyme, then cool to room temperature, add enzyme solution and water, stir evenly, and enzymolyze for 0.5-2.0h; (3) add ethanol and water to the mixture of tea seed powder after enzymolysis, so that the ratio of material to liquid is 1:25, the ethanol concentration is 60%; adjust the pH value to 1.5-2.0, reflux extraction for 25-40min, then centrifuge, take out the centrifuged supernatant for later use; wash the filter residue with 60% ethanol, and then adjust the pH to 7 ‑7.5 and 8.0‑8.5 repeat the above steps of reflux extraction and centrifugation; combine the centrifugates three times, rotovap and dry to obtain the target product. In the present invention, the yield of total flavonoids of tea seed powder is much higher than that of the prior art by combining enzymatic-semi-bionic technology, which shows that the extraction of flavonoids from tea seed powder by this method has obvious advantages.

Description

technical field [0001] The invention belongs to the field of extraction of flavonoids, and in particular relates to a method for extracting flavonoids in tea seeds through an aqueous enzymatic method combined with a semi-bionic method. Background technique [0002] my country is a big tea-producing country with the largest planting area and output in the world. It is rich in tea tree resources and has an annual output of more than 800 million kilograms of tea seeds. However, people have underdeveloped and utilized tea seeds for a long time, and most of the tea seeds have been wasted. Now, the research on tea seeds at home and abroad is mainly focused on the extraction and refining of tea seed oil and the extraction of tea saponin, while the research and development of flavonoids is less. It is of great significance to develop tea seed flavonoids and avoid waste of resources. and broad prospects. [0003] The extraction of flavonoids by aqueous enzymatic method is a relatively...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07G99/00
Inventor 王晓琴林莉
Owner HUAQIAO UNIVERSITY