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Molecular breeding method for synchronously improving length, strength, and fineness of cotton fiber through polymerizing chromosome segment introgression lines

A technique for chromosome fragmentation and fiber strength, applied in botany equipment and methods, biochemical equipment and methods, plant genetic improvement, etc., to achieve the effects of long breeding cycle, increased fiber length and fiber strength, and heavy hybridization workload

Active Publication Date: 2014-01-15
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, the offspring of the two hybrids showed the typical crazy separation of interspecific hybridization. Cotton breeders have been trying to breed new varieties with high yield of upland cotton and high-quality sea-island cotton, but have not been successful.

Method used

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  • Molecular breeding method for synchronously improving length, strength, and fineness of cotton fiber through polymerizing chromosome segment introgression lines
  • Molecular breeding method for synchronously improving length, strength, and fineness of cotton fiber through polymerizing chromosome segment introgression lines
  • Molecular breeding method for synchronously improving length, strength, and fineness of cotton fiber through polymerizing chromosome segment introgression lines

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Embodiment 1

[0028] Selected parents: The Cotton Research Institute of the State Key Laboratory of Crop Genetics and Germplasm Innovation of Nanjing Agricultural University uses the genetic standard line TM-1 of upland cotton as the recurrent parent, and the disease-resistant and high-quality Sea Island Cotton Sea 7124 strain as the donor parent (non-recurrent parent) , combined with SSR molecular marker-assisted selection covering the whole genome in the high generation of backcrossing, 174 sea-island cotton chromosome fragment introduction lines with the background of TM-1, the first domestic upland cotton genetic standard line, were bred, and the genome coverage rate reached 83.5%. Through continuous 2-year testing of cotton fiber quality of sea-island cotton chromosome segment introduction lines, the introduction lines IL080‐D6‐1 (CGMCC NO.4685) and 1047 (CGMCC NO. ‐A2‐6 chromosome fragment introduction line). IL080‐D6‐1 fiber length 31.5‐32.5mm, fiber strength 32.7‐33.5cN / tex, microna...

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Abstract

The invention belongs to the field of molecular breeding, and relates to a molecular breeding method for synchronously improving the length, strength, and fineness of cotton fiber through polymerizing chromosome segment introgression lines. The method comprises the following steps: taking two introgression lines, namely IL080-D6-1 and 1047 (IL019-A2-6 chromosome segment introgression line), as the non-recurrent parents, respectively subjecting the non-recurrent parents to carry out hybridization with a recurrent parent (Xinjiang 0768) for one time, backcrossing two generations, in BC2F1 making strains containing different chromosome segments intercross with each other so as to obtain double-segment integrated F2 segregation population, identifying the double-segment homozygous strains through each two molecular labels on two segments, carrying out field tests under four circumstances, and keeping on selfing for two generations so as to obtain a double-segment homozygous novel cotton breed, whose fiber length, fiber strength and fineness have been prominently improved. A high-quality and high-yield novel cotton species, named "Nannong 08-66", has been cultured by utilizing the method provided by the invention.

Description

technical field [0001] The invention belongs to the field of molecular breeding, and relates to a molecular breeding method for synchronously improving cotton fiber length, strength and fineness by introducing lines of polymerized chromosome fragments. Background technique [0002] Cotton is an important economic crop worldwide. China is a big cotton producer, consumer and textile country. As a major cotton-producing country, my country's main cotton-producing areas cover 16 provinces, municipalities (autonomous regions), with an annual cotton planting area of ​​70-80 million mu, with a total output of about 6 million tons, accounting for a quarter of the world's total output. With the advancement of textile technology, textile products have also developed from coarse-count yarns to high-count yarns and even fine sands of 100 counts, and the processing objects have also developed from ordinary medium-staple cotton to medium-long-staple cotton, long-staple cotton and super-l...

Claims

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Application Information

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IPC IPC(8): C12Q1/68A01H1/02A01H1/04A01H5/00
CPCA01H1/02A01H1/04C12Q1/6895C12Q2600/156
Inventor 张天真曹志斌王鹏
Owner NANJING AGRICULTURAL UNIVERSITY
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