Antipodal kauri alkane glycoside compounds as well as preparation method and applications thereof

A technology of kauritanes and compounds, which is applied in the field of medicine, can solve the problems of rare research reports and few research reports on the active chemical components of chrysanthemum berries, and achieve broad application prospects and the effect of improving activity

Inactive Publication Date: 2014-01-22
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there are not many research reports on the active chemical constituents of mulberry
Moreover, in previous studies, researchers are usually used to using the root of t

Method used

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  • Antipodal kauri alkane glycoside compounds as well as preparation method and applications thereof
  • Antipodal kauri alkane glycoside compounds as well as preparation method and applications thereof
  • Antipodal kauri alkane glycoside compounds as well as preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1. Extraction, separation and identification of ent-kaurene glycoside compounds

[0021] 1. Extraction and separation

[0022] a. Extraction: dry and pulverize the stems and leaves of the strawberry, and divide them into two parts on average, one of which uses ethanol with a concentration of 65% by volume as a solvent, and extracts it by reflux for 3 times, adding an amount equivalent to the stems and leaves of the strawberry each time. 17 times the total weight of solvent extraction for 1 hour, collect the extract; another part uses ethanol with a volume percentage concentration of 65% as a solvent for percolation, and collects the percolation liquid; combines the extraction liquid and the percolation liquid, and recovers ethanol under reduced pressure , to prepare liquid extract;

[0023] b. Extraction: Disperse the liquid extract obtained in step a in water, extract with petroleum ether, water-saturated ethyl acetate, and water-saturated n-butanol successive...

Embodiment 2

[0036] Example 2. Research on anti-inflammatory activity of ent-kaurene-type glycoside compounds

[0037] Collect mouse peritoneal macrophage RAW264.7 in the logarithmic growth phase, and use 1×10 5cells / mL were inoculated in 96-well cell culture plates, and divided into four experimental groups: simple endotoxin lipopolysaccharide (LPS) stimulation group, low concentration compound treatment group (20 μg / mL), high concentration compound treatment group (50 μg / mL) mL) and the blank control group, with three replicate wells for each group. After the cells adhered to the wall, the low-concentration and high-concentration compound treatment groups were respectively added corresponding concentrations of enantio-kaurane-type glycoside compounds with the structural formula I or II, and incubated for 2 hours. Then, the simple endotoxin LPS stimulation group, low-concentration LPS with a final concentration of 10 pg / mL was added to the treatment group and high-concentration compound ...

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Abstract

The invention discloses two novel antipodal kauri alkane glycoside compounds separated from stems and leaves of rubus parvifolius, wherein the structural formulas of the two compounds are respectively shown as I and II. The in-vitro anti-inflammatory activity research shows that the two compounds have an obvious protection effect to inflammation induced by LPS (lipoply saccharide), can be used for preparing anti-inflammatory medicaments and anti-inflammatory health-care products, and can also be used as chemical synthesis intermediates and the like.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to two novel enantio-kaurane-type glycoside compounds separated from the stems and leaves of Mao berry, and also relates to the extraction and separation method of these two compounds, as well as their use in medicine and health care . Background technique [0002] Maoberry Rubus parvifolius L. is a small deciduous shrub of the genus Rubus in the family Rosaceae. It grows in hillside miscellaneous forests, sunny valleys, roadsides or wilderness at an altitude of 400-2600 meters. It is widely distributed in Heilongjiang, Jilin, Liaoning, Hebei, Shanxi, Shaanxi, Gansu, Shandong, Jiangsu, Anhui, Zhejiang, Jiangxi, Fujian, Taiwan, Henan, Hubei, Hunan, Guangdong, Guangxi, Sichuan, Guizhou and other places are rich in wild resources. Its roots, stems, and leaves are used as medicine, and its medicinal name is usually "Tianxiu". Modern research shows that Mao berry has various pharmacolog...

Claims

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Application Information

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IPC IPC(8): C07H15/18C07H1/08A61K31/7032A61P29/00
Inventor 陈剑鸿胡小刚张宏伟夏培元黄明春蔡永青戴青
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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