Method for preparing ganoderma vinegar from ganoderma submerged broth
A technology of deep fermentation and fermentation liquid, which is applied in the preparation of vinegar and other directions, can solve the problem of not completely solving bitterness and other problems, and achieve the effects of retaining medicinal value, significant economic benefits, and sour taste.
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Embodiment 1
[0025] The method for preparing Ganoderma lucidum vinegar from Ganoderma lucidum submerged broth:
[0026] (1) Repurchase Ganoderma lucidum strain with bacterial number 5.0065 from the General Microbiology Center of China Microbiological Culture Collection Management Committee, and keep it for later use;
[0027] (2) Preparation of PDA slant solid medium The preparation method of PDA slant solid medium is: when preparing 1000mL of PDA slant solid medium, weigh 200g of peeled potatoes, cut into pieces with a side length of 2-3cm, add them to 1000mL of water, and boil After 30 minutes, filter with 4 layers of gauze, add water to 1000mL of the filtrate, then add 20g of glucose and 20g of agar, heat and melt, then pack into containers, sterilize at 121°C for 20 minutes, and cool down;
[0028] (3) Inoculate the Ganoderma lucidum strain of bacterial number 5.0065 on the PDA slant solid medium prepared in (2), and cultivate it at 28° C. for 6 days, and the slant solid medium is cov...
Embodiment 2
[0035] The method for preparing Ganoderma lucidum vinegar from Ganoderma lucidum submerged broth:
[0036] (1) Purchasing strains, preparing PDA slant solid medium, preparing potato juice, preparing primary and secondary fermentation broths are the same as in Example 1;
[0037] (2) Inoculate the Ganoderma lucidum strain with bacterial number 5.0065 on the PDA slant solid medium prepared in (1), and cultivate it at 29°C for 6 days, the slant solid medium is covered with mycelia, and set aside;
[0038] (3) Put the primary fermentation culture liquid prepared in (2) into the conical flasks respectively, and put 100mL in each bottle. Pick 4 pieces of bacterial blocks with a side length of 5 mm from the culture medium, insert them into the primary fermentation culture medium, and then place them on a constant temperature shaking shaker for culture, the shaker speed is 160r / min, and cultivate at 26°C for 7 days. When it is clear and many uniform small mycelium balls are formed,...
Embodiment 3
[0042] The method for preparing Ganoderma lucidum vinegar from Ganoderma lucidum submerged broth:
[0043] (1) Purchasing strains, preparing PDA slant solid medium, preparing potato juice, preparing primary and secondary fermentation broths are the same as in Example 1;
[0044] (2) Inoculate the Ganoderma lucidum strain with bacterial number 5.0065 on the PDA slant solid medium prepared in (1), and cultivate it at 30°C for 7 days, and the slant solid medium is covered with hyphae, and set aside;
[0045] (3) Put the primary fermentation culture liquid prepared in (2) into the conical flasks respectively, and put 100mL in each bottle. Pick 5 pieces of bacterial blocks with a side length of 3 mm from the culture medium, insert them into the primary fermentation culture medium, and then place them on a constant temperature shaking shaker for cultivation. The shaker speed is 180r / min. When it is clear and many uniform small mycelium balls are formed, it is made into the primar...
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