Aflatoxin B1 detection method based on three-dimensional fluorescence

Abstract

The invention discloses an aflatoxin B1 detection method based on three-dimensional fluorescence and belongs to the technical field of environment detection. The method comprises the following two parts: based on a parallel factor analysis method (PARAFAC), according to a standard sample, establishing a quantitative analysis model and quantitatively detecting a to-be-detected sample, specifically, based on the PARAFAC, the establishment of the quantitative analysis model according to the standard sample comprises the steps of preparing the standard sample, preprocessing the standard sample, quantitatively analyzing the standard sample by adopting a standard method, determining the EEM chromatogram characteristic area of the standard sample and establishing a quantitative analysis model of the standard sample; quantitative detection on the to-be-detected sample specifically comprises the steps of preprocessing the to-be-detected sample, selecting the EEM characteristic area, performing EEM measurement and calculating the measurement value according to the quantitative analysis model. The aflatoxin B1 detection method based on three-dimensional fluorescence has the advantages of being fast in detection speed, high in sensitivity, high in accuracy, good in quantifying effect, and the like, and is suitable for detecting the aflatoxin in agricultural products.

Description

Technical field [0001] Based on three -dimensional fluorescence detection of machinicillin B 1 The method is the technical field of environmental detection. Background technique [0002] Cuterocycin is a very toxic and toxic substance. In 1993, it was designated by the Cancer research institution of the World Health Organization (WHO) as type 1 carcinogen.In addition to carcinogenicity, patelomycin also has a destructive effect on human visceral tissues, and its toxicity is much higher than cyanide, arsenidies and organic pesticides.Among them, pornomycin B 1 It exists in naturally polluted foods, such as corn, peanuts, cotton seeds, and dried fruits, which are more likely to be eaten by mistake.When ingestion of trace macrobacterin B 1 , Can cause chronic poisoning, growth disorders, causing fibrous lesions, and causing fibrous tissue to hyperplasia; as the intake increases, acute poisoning can be caused, acute hepatitis, hemorrhagic necrosis, hepatocyte fat degeneration and bil...

AI Technical Summary

Problems solved by technology

This method has good reproducibility and can realize quantitative analysis; unfortunately, this method can only extract the convex set area containing useful spectral information from the spectrogram, but it does not solve the background spectrum and background drift in the three-dimensional fluorescence spectrum , so it does not improve the accuracy of the concentration fluorescence peak intensity obtained from the original fluorescence spectrum, that is, it does not improve the use of fluorescence peak intensity to judge aflatoxin B 1 Content accuracy

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