Aflatoxin B1 detection method based on three-dimensional fluorescence

An aflatoxin, three-dimensional fluorescence technology, applied in the direction of fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of not solving the background spectrum and background drift, not improving the accuracy, not improving the accuracy of the concentration fluorescence peak intensity, etc. Achieve the effect of eliminating baseline drift, good quantitative effect and fast detection speed

Inactive Publication Date: 2014-01-22
INNER MONGOLIA ZHENGLONG GRAIN FOOD
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Problems solved by technology

This method has good reproducibility and can realize quantitative analysis; unfortunately, this method can only extract the convex set area containing useful spectral information from the spectrogram, but it does not solve the background spectrum and background drift in the three

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  • Aflatoxin B1 detection method based on three-dimensional fluorescence
  • Aflatoxin B1 detection method based on three-dimensional fluorescence
  • Aflatoxin B1 detection method based on three-dimensional fluorescence

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specific Embodiment 2

[0068] The sample detected in this embodiment is soy sauce containing aflatoxin.

[0069] The three-dimensional fluorescent detection of aflatoxin B based on the present embodiment 1 method, including the following steps:

[0070] a. Based on the parallel factor analysis method (PARAFAC), the quantitative analysis model is established according to the standard sample, which is the same as the specific example 1, specifically:

[0071] Step a1, preparing standard samples;

[0072] Step a2, pretreating the standard sample;

[0073] Step a3, using a standard method to quantitatively analyze the standard sample;

[0074] Step a4, using a fluorescence spectrophotometer to measure the standard sample to determine the characteristic area of ​​the EEM spectrum of the standard sample;

[0075] Step a5, establishing a quantitative analysis model for standard samples based on PARAFAC;

[0076] B, quantitative detection is carried out on the sample to be tested, which is the same as ...

specific Embodiment 3

[0097] The samples tested in this embodiment are peanuts containing aflatoxin.

[0098] The three-dimensional fluorescent detection of aflatoxin B based on the present embodiment 1 method, including the following steps:

[0099] a. Based on the parallel factor analysis method (PARAFAC), the quantitative analysis model is established according to the standard sample, which is the same as the specific example 1, specifically:

[0100] Step a1, preparing standard samples;

[0101] Step a2, pretreating the standard sample;

[0102] Step a3, using a standard method to quantitatively analyze the standard sample;

[0103] Step a4, using a fluorescence spectrophotometer to measure the standard sample to determine the characteristic area of ​​the EEM spectrum of the standard sample;

[0104] Step a5, establishing a quantitative analysis model for standard samples based on PARAFAC;

[0105] B, quantitative detection is carried out on the sample to be tested, which is the same as th...

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Abstract

The invention discloses an aflatoxin B1 detection method based on three-dimensional fluorescence and belongs to the technical field of environment detection. The method comprises the following two parts: based on a parallel factor analysis method (PARAFAC), according to a standard sample, establishing a quantitative analysis model and quantitatively detecting a to-be-detected sample, specifically, based on the PARAFAC, the establishment of the quantitative analysis model according to the standard sample comprises the steps of preparing the standard sample, preprocessing the standard sample, quantitatively analyzing the standard sample by adopting a standard method, determining the EEM chromatogram characteristic area of the standard sample and establishing a quantitative analysis model of the standard sample; quantitative detection on the to-be-detected sample specifically comprises the steps of preprocessing the to-be-detected sample, selecting the EEM characteristic area, performing EEM measurement and calculating the measurement value according to the quantitative analysis model. The aflatoxin B1 detection method based on three-dimensional fluorescence has the advantages of being fast in detection speed, high in sensitivity, high in accuracy, good in quantifying effect, and the like, and is suitable for detecting the aflatoxin in agricultural products.

Description

Technical field [0001] Based on three -dimensional fluorescence detection of machinicillin B 1 The method is the technical field of environmental detection. Background technique [0002] Cuterocycin is a very toxic and toxic substance. In 1993, it was designated by the Cancer research institution of the World Health Organization (WHO) as type 1 carcinogen.In addition to carcinogenicity, patelomycin also has a destructive effect on human visceral tissues, and its toxicity is much higher than cyanide, arsenidies and organic pesticides.Among them, pornomycin B 1 It exists in naturally polluted foods, such as corn, peanuts, cotton seeds, and dried fruits, which are more likely to be eaten by mistake.When ingestion of trace macrobacterin B 1 , Can cause chronic poisoning, growth disorders, causing fibrous lesions, and causing fibrous tissue to hyperplasia; as the intake increases, acute poisoning can be caused, acute hepatitis, hemorrhagic necrosis, hepatocyte fat degeneration and bil...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 赵春城赵烟桥
Owner INNER MONGOLIA ZHENGLONG GRAIN FOOD
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