Tissue culture medium of camellia azalea and propagation method thereof
A technology of Rhododendron red camellia and tissue culture medium, which is applied in the field of plant tissue culture, can solve the problems of low reproduction rate, short supply, slow reproduction speed and the like, and achieves the effects of high bud induction rate, developed root system and high plant extraction.
Inactive Publication Date: 2014-12-31
CHONGQING UNIV OF ARTS & SCI
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Problems solved by technology
In recent years, Guangdong, Zhejiang and other places have gradually become market-oriented, but all of them are obtained through traditional grafting and cuttings. The reproduction speed is slow and the reproduction rate is low, which still cannot meet the needs of the market.
Using tissue culture technology to propagate Rhododendron red camellia can effectively solve the problem that the market is in short supply, but there is no relevant report on the technology of using tissue culture technology to propagate Rhododendron red camellia
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Embodiment Construction
[0015] Preferred embodiments of the present invention will be described in detail below.
[0016] The test materials used in the examples of the present invention are young and tender stem tips of Rhododendron red camellia provided by Chongqing Nanshan Botanical Garden, and the collection time was mid-April 2010.
[0017] 1) Induction of buds: In mid-April, select 3-5cm long young shoot tips of the year as explants, wash them with tap water first, cut off the leaves, leave the shoot tips 2-3cm, add a little washing powder to dissolve Soak the cut material for 5-10 minutes to remove surface dust, then rinse it with running water, turn on the ultraviolet lamp on the ultra-clean workbench to dry, put it in 0.1% mercury liter for sterilization for 5-6 minutes, and then rinse it with sterile water 4 to 5 times, cut off the base of the shoot tip, so that the shoot tip is controlled at about 1cm in length; inoculate the shoot tip of the obtained sterile material into induction media ...
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Abstract
The invention discloses a tissue culture medium of camellia azalea and a propagation method of the tissue culture medium. The tissue culture medium comprises an inducing culture medium, a proliferation culture medium and a rooting culture medium. The method using the tissue culture medium of the camellia azalea to propagate the camellia azalea comprises the three steps of induction of sprouts, proliferation culture of the sprouts and rooting culture. The tissue culture medium and the propagation method have the beneficial effects that a method for propagating the camellia azalea by a tissue culture technology is established, and the inducing culture medium, the proliferation culture medium and the rooting culture medium in the method are selected and optimized; and the tissue culture medium has the advantages of high sprout induction rate, high proliferation coefficient, high rooting rate, fast plant heightening, normal leaf shape and color and the like, can meet the need of the market for the camellia azalea, and is low in cost and stable in character.
Description
technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a tissue culture medium of rhododendron camellia and a propagation method thereof. Background technique [0002] Camellia azalea got its name because it resembles a cuckoo in appearance but is actually a camellia. Rhododendron red camellia belongs to the family Theaceae and belongs to the genus Camellia, and its cultivation name is "Four Seasons Rhododendron tea" or "Rhododendron tea". Rhododendron red camellia is an extremely rare species of camellia, known as the "giant panda in the plant kingdom", once endangered Rhododendron red camellia. It is mainly distributed in Yunnan, Guangxi, Guangdong, and Sichuan. It is rare in the wild and rare in the camellia market. It is currently in the research and development stage and is one of the precious ancient camellia varieties that bloom in four seasons. In recent years, Guangdong, Zhejiang and other places have been gradu...
Claims
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IPC IPC(8): C12N5/04A01H4/00
Inventor 陈泽雄娄娟
Owner CHONGQING UNIV OF ARTS & SCI