Method for establishing high altitude cerebral edema animal model

A high-altitude cerebral edema, animal model technology, applied in animal husbandry and other directions, can solve the problems of difficult quantification and automatic control, poor repeatability, and insignificant symptoms induced by cerebral edema, and achieve increased oxygen consumption, enhanced respiratory rate and oxygen consumption. amount of effect

Inactive Publication Date: 2014-01-29
CHENGDU MILITARY GENERAL HOSPITAL OF PLA
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Problems solved by technology

This modeling method has many disadvantages: First, the experimental rats have strong adaptability. If a low-pressure hypoxic environment comparable to that in the plateau area is used, t

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  • Method for establishing high altitude cerebral edema animal model
  • Method for establishing high altitude cerebral edema animal model
  • Method for establishing high altitude cerebral edema animal model

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Select 20 clean-grade Vistar male rats, and the weight of each rat is controlled at 185g-200g. The rats were placed in a hypobaric and hypoxic environment to move freely for 2 days, and the conditions of the hypobaric and hypoxic environment were as follows: the temperature was 4-7 degrees Celsius, the oxygen concentration was 0.020 mol / L, and the air pressure was 45KPa. Under this environment, the rats were given a normal amount of food and drink.

[0023] Two days later, mice were trained to swim under the same hypobaric hypoxic conditions. Swimming training lasted 4 days, with 6 swimming training sessions per day. The process of each swimming training is as follows: the rats swim in the swimming pool for 20 minutes under low pressure and hypoxic conditions, and then the rats rest for 15 minutes; Continue to swim for 20 minutes and then rest for 15 minutes; repeat this, practice 6 times a day.

[0024] After the 6-day experiment, the rats were identified and monito...

Embodiment 2

[0026] Select 20 clean-grade Vistar male rats, and the weight of each rat is controlled at 200g-215g. Rats were placed in a hypobaric hypoxic environment to move freely for 2 days, and the conditions of the hypobaric hypoxic environment were: temperature 7-10 degrees Celsius, oxygen concentration 0.020mol / L-0.027mol / L, air pressure 50KPa. Under this environment, the rats were given a normal amount of food and drink.

[0027] Two days later, mice were trained to swim under the same hypobaric hypoxic conditions. Swimming training lasted 6 days, with 6 swimming training sessions per day. The process of each swimming training is as follows: the rats swim in the swimming pool for 30 minutes under low pressure and hypoxic conditions, and then the rats rest for 25 minutes; Continue to swim for 30 minutes and then rest for 25 minutes; repeat this, practice 6 times a day.

[0028] After the 8-day experiment, the rats were identified and monitored for the symptoms of brain edema by u...

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Abstract

The invention discloses a method for establishing a high altitude cerebral edema animal model. The method for establishing the high altitude cerebral edema animal model comprises the following steps of selecting a plurality of rats, putting the rats in a low-pressure low-oxygen environment for two days with normal diets, continuously carrying out 6 times of swimming training on the rats every day for consecutive 4-6 days, maintaining the normal diets everyday, and establishing the high altitude cerebral edema animal model, wherein a method for the swimming training comprises the steps that the rats are put into a swimming pool to swim freely, the low-pressure low-oxygen environment is maintained, the rats rest for 15-25 minutes after the rats swim for 20-30 minutes every time, and then the rats are immediately made to proceed with the swimming training of a next time. The rats can be induced to generate cerebral edema symptoms in the low-pressure low-oxygen environment; multiple times of the swimming training is carried out on the rats in several consecutive days in the low-pressure low-oxygen environment condition, the breathing frequency and the oxygen consumption of the rats are enhanced in the swimming training process, the cerebral edema symptoms of the rats are made to become obvious by increasing the oxygen consumption of the rats, and it is detected in the subsequent detection that the positive rate of cerebral edemas is increased.

Description

technical field [0001] The invention relates to a modeling method of an animal model, in particular to a method for constructing an animal model of plateau cerebral edema. Background technique [0002] High-altitude cerebral edema is a severe dysfunction of the central nervous system caused by acute hypoxia. It is characterized by acute onset, and clinical manifestations are characterized by severe headache, vomiting, ataxia, and progressive disturbance of consciousness. Pathological changes mainly include cerebral ischemia or hypoxic injury, cerebral circulation disorder, resulting in cerebral edema and increased intracranial pressure. If not treated properly, often life-threatening. In the past, it was often called alpine (original) coma, cerebral mountain sickness, acute mountain sickness encephalopathy, and high altitude cerebral hypoxia syndrome. [0003] The pathological essence of high-altitude cerebral edema is cerebral edema, clinically manifested as a series of ...

Claims

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Application Information

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IPC IPC(8): A01K67/02
Inventor 张艳超顾建文树海峰张俊海赵凯林龙范珂夏
Owner CHENGDU MILITARY GENERAL HOSPITAL OF PLA
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