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Preparation method for three-dimensional mineralized collagen bracket with strict grade structure as well as product and application of three-dimensional mineralized collagen bracket

A technology of mineralized collagen and hierarchical structure, which is applied in the preparation of three-dimensional mineralized collagen scaffolds, the preparation of mineralized collagen scaffolds, and the application fields in the preparation of bone tissue repair materials

Inactive Publication Date: 2014-11-05
诚靓科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mineralized collagen synthesized in the traditional way only achieves the same chemical composition as bone tissue, and does not replicate its hierarchical structure

Method used

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  • Preparation method for three-dimensional mineralized collagen bracket with strict grade structure as well as product and application of three-dimensional mineralized collagen bracket
  • Preparation method for three-dimensional mineralized collagen bracket with strict grade structure as well as product and application of three-dimensional mineralized collagen bracket
  • Preparation method for three-dimensional mineralized collagen bracket with strict grade structure as well as product and application of three-dimensional mineralized collagen bracket

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Preparation of a three-dimensional mineralized collagen scaffold with strict hierarchical structure

[0048] (1) Collagen phosphorylation: Soak the dialysis bag containing 3 mg / ml rat tail type I procollagen solution in 1.25% sodium tripolyphosphate solution for 5 minutes to phosphorylate the collagen;

[0049] (2) Preparation of amorphous calcium phosphate (ACP): Soak 0.5g of type I white silicate cement in 30ml of artificial simulated body fluid containing 0.5mg / ml polyacrylic acid, react at 37°C for 1d, and obtain a cement with a size of 45 -50nm nano-amorphous calcium phosphate solution;

[0050] (3) Collagen fibrosis and remineralization: put the phosphorylated collagen obtained in step (1) into the ACP-containing solution obtained in step (2), the ratio of collagen mass to solution volume is 1mg: 1ml, after 5 days , the phosphorylated collagen will induce the orderly arrangement and nucleation of nano-amorphous calcium phosphate in collagen fibers, form...

Embodiment 2

[0053] Example 2 Preparation of three-dimensional mineralized collagen scaffold with strict hierarchical structure

[0054] (1) Collagen phosphorylation: Soak the dialysis bag containing 4 mg / ml rat tail type I procollagen solution in 2.5% sodium tripolyphosphate solution for 10 minutes to phosphorylate the collagen;

[0055] (2) Preparation of amorphous calcium phosphate (ACP): Soak 1g of type I white silicate cement in 40ml of artificial simulated body fluid containing 1mg / ml of polyacrylic acid, react at 37°C for 2 days, and obtain a cement with a size of 45-50nm A solution of nanometer amorphous calcium phosphate;

[0056] (3) Fibrosis and remineralization of collagen: place the phosphorylated collagen obtained in step (1) in the ACP-containing solution obtained in step (2), the ratio of collagen mass to solution volume is 0.5mg: 1ml, 7d Finally, the phosphorylated collagen will induce the orderly arrangement and nucleation of nano-amorphous calcium phosphate in collagen ...

Embodiment 3

[0059] Example 3 Preparation of Three-dimensional Mineralized Collagen Scaffold with Strict Hierarchical Structure

[0060] (1) Collagen phosphorylation: Soak the dialysis bag containing 3.5 mg / ml rat tail type I procollagen solution in 2% sodium tripolyphosphate solution for 10 minutes to phosphorylate the collagen;

[0061] (2) Preparation of amorphous calcium phosphate (ACP): Soak 1g of type I white silicate cement in 30-40ml of artificial simulated body fluid containing 1mg / ml polyacrylic acid, react at 37°C for 36h, and obtain 45 -50nm nano-amorphous calcium phosphate solution;

[0062] (3) Fibrosis and remineralization of collagen: place the phosphorylated collagen obtained in step (1) in the ACP-containing solution obtained in step (2), the ratio of collagen mass to solution volume is 1mg: 1ml, after 6 days , the phosphorylated collagen will induce the ordered arrangement and nucleation of nano-amorphous calcium phosphate in the fiber, forming mineralized collagen with...

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Abstract

The invention discloses a preparation method for a three-dimensional mineralized collagen bracket with a strict grade structure as well as a product and an application of the three-dimensional mineralized collagen bracket. The three-dimensional mineralized collagen bracket with the strict grade structure, provided by the invention, not only copies chemical components (Ca / P=1.67) of natural bone tissues; more importantly, the three-dimensional mineralized collagen bracket also reappears the grade structure of natural bone mineralized collagen, namely a periodic transverse line structure (D=67nm) formed in fibers by nano plate-shaped crystals. According to the novel mineralized collagen bracket with the strict grade structure, the mechanical property and the biocompatibility (cell proliferation, differentiation and osteogenic capability) in dry and wet states are better than those of conventional mineralized collagen. Furthermore, the mechanical property (13.7+ / -2.6GPa) of the novel mineralized collagen bracket with the strict grade structure is similar with that of the bone tissues. Therefore, the three-dimensional mineralized collagen bracket with the strict grade structure has a wide application prospect in the field of preparing bone tissue repairing materials.

Description

technical field [0001] The invention relates to a preparation method of a mineralized collagen scaffold, in particular to a preparation method of a three-dimensional mineralized collagen scaffold with a strict hierarchical structure, its product and its application in the preparation of bone tissue repair materials. It belongs to the field of bone tissue repair and treatment. Background technique [0002] Bone tissue is a kind of strictly hierarchical structure and multifunctional mineralized tissue in nature. During life, it is constantly remodeled to adapt to external mechanical stress, maintain ionic balance and repair defects. The regeneration and repair of bone defects has always been an important topic in medical research. Autologous bone grafting has long been considered the gold standard for the treatment of bone defects. However, it was questioned due to the limited source and the need for secondary procedures for the parts obtained. In recent years, tissue engi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/24A61L27/12A61L27/56
Inventor 刘燕周彦恒刘帅付玉
Owner 诚靓科技有限责任公司