Method for constructing and cultivating salt-tolerant lawn grass

A turfgrass and salt-tolerant technology, which is applied in the field of building and cultivating salt-tolerant turfgrass, can solve the problems of a large amount of initial investment and achieve the effect of less investment

Inactive Publication Date: 2014-01-29
NINGXIA UNIVERSITY
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Problems solved by technology

But all of these methods require a significant upfront investment

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  • Method for constructing and cultivating salt-tolerant lawn grass
  • Method for constructing and cultivating salt-tolerant lawn grass
  • Method for constructing and cultivating salt-tolerant lawn grass

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Embodiment Construction

[0030] The present invention will be specifically introduced below in conjunction with the accompanying drawings and specific embodiments.

[0031] refer to figure 1 , the method for constructing and cultivating salt-tolerant lawn grass of the present invention, comprises the following steps:

[0032] (1) Construction of Agrobacterium recombinant binary plasmids: Cloning the complete expression genes of Arabidopsis SOS1, SOS2, SOS3, and CBL10, and implanting them into E. coli binary plasmids to obtain recombinant binary plasmids;

[0033] (2) Transformation of Agrobacterium and turfgrass: introducing the recombinant binary plasmid into Agrobacterium and infecting turfgrass callus;

[0034] (3) Select and cultivate transgenic lawn grass.

[0035] The following uses tall fescue as an example to illustrate, and the operation of other lawn grasses is the same.

[0036] First, the complete coding sequence of Arabidopsis SOS gene was amplified from its cDNA clone by PCR.

[0037...

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Abstract

The invention discloses a method for constructing and cultivating salt-tolerant lawn grass. The method is characterized by comprising the following steps of (1) constructing recombinant binary plasmids for agrobacterium tumefaciens, namely performing complete expressed gene cloning on Arabidopsis thaliana SOS1, SOS2, SOS3 and CBL10, and implanting expressed genes into binary plasmids of Escherichia coli to obtain the recombinant binary plasmids; (2) transforming the agrobacterium tumefaciens and lawn grass, namely introducing the recombinant binary plasmids into the agrobacterium tumefaciens, and infecting callus tissues of the lawn grass; and (3) selecting and cultivating transgenic lawn grass. The method has the beneficial effects that a new way for the improvement of saline-alkali soil is provided by the construction and the cultivation of the transgenic salt-tolerant lawn grass, and saline-alkali soil improvement engineering is upgraded into high and new technological genetic engineering; the method is less in input, and has considerable long-term effects due to the adoption of asexual reproduction.

Description

technical field [0001] The invention relates to a method for constructing and cultivating salt-tolerant turfgrass, which belongs to the technical field of biological transgenics. Background technique [0002] At present, there are 1.8 billion mu of arable land in my country, and the area of ​​saline-alkali land accounts for 540 million mu. The western region of my country is located in an arid and semi-arid area. Due to drought and water shortage, salinization and desertification are serious. At the same time, due to climate reasons, global warming and human activities in recent years, the change in the national cultivated land area has generally shown a downward trend. This phenomenon is also becoming more and more serious, and has become a worldwide problem that needs to be solved urgently. [0003] The existing main methods to improve saline-alkali land are to build a large number of drainage networks to make the surface salt flow into the lower places with irrigation wat...

Claims

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Application Information

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IPC IPC(8): C12N15/84A01H5/00
Inventor 麻冬梅许兴郭伶娜蔡进军杨亚亚
Owner NINGXIA UNIVERSITY
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