Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of phyllostachys pubescens leaf extract

A moso bamboo and extraction technology is applied in the directions of pharmaceutical formulations, plant raw materials, medical preparations containing active ingredients, etc., and can solve the problems of missing total flavonoids from bamboo leaves, large amount of solvent, and long extraction time, so as to improve labor efficiency, Less solvent consumption and good reproducibility

Inactive Publication Date: 2014-02-05
江西省药物研究所
View PDF2 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the method for extracting flavonoids from bamboo leaves has disadvantages such as long extraction time, large amount of solvent used, and high energy consumption. There is no related patent on the preparation method of total flavonoids from bamboo leaves in China

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The dried moso bamboo leaves were crushed, weighed 1kg, put into the extraction tank, and use absolute ethanol as the carrier, the carrier amount was 25% of the extracted material, the temperature of the extraction kettle was 40℃, and the temperature of the analysis kettle was 35℃. The pressure of the kettle is 30MPa, CO 2 The flow rate is 25L / h. After 3 hours of extraction, the extract is desorbed. The pressure of the desorption vessel is 6MPa. The extract is dispersed in water to obtain 1.0g crude drug / ml sample solution, which is dynamically adsorbed by 250gD101 macroporous adsorption resin. After the adsorption is completed Wash with water until the color is light, and then eluted with 70% ethanol. The eluate is concentrated under reduced pressure, and the concentrated solution is adsorbed by 250g polyamide resin. After the adsorption is completed, wash with water and then eluted with 50% ethanol. Collect the ethanol The eluate is concentrated under reduced pressure, ...

Embodiment 2

[0019] The dried moso bamboo leaves are crushed, weighed out 1kg, put into the extraction tank, and use absolute ethanol as the carrier, the carrier amount is 30% of the extracted material, the temperature of the extraction kettle is 35°C, the temperature of the analysis kettle is 30°C, extraction The pressure of the kettle is 35MPa, CO 2 The flow rate is 30L / h. After 4 hours of extraction, the extract is desorbed. The pressure of the desorption tank is 6MPa. The extract is dispersed in water to obtain a sample solution of 0.9g crude drug / ml, which is dynamically adsorbed by 250gD101 macroporous adsorption resin. Wash with water until the color is light, and then eluted with 70% ethanol. The eluate is concentrated under reduced pressure, and the concentrated solution is adsorbed by 250g polyamide resin. After the adsorption is completed, wash with water and then eluted with 60% ethanol. Collect the ethanol The eluate is concentrated under reduced pressure, and the concentrate is...

Embodiment 3

[0021] The dried moso bamboo leaves are crushed, weighed 1kg, put into the extraction tank, and use absolute ethanol as the carrier, the carrier amount is 20% of the extracted material, the temperature of the extraction kettle is 35°C, the temperature of the analysis kettle is 30°C, extraction The kettle pressure is 30MPa, the analytical kettle pressure is 6.5MPa, CO 2 The flow rate is 20L / h, and the extraction time is 3h. The extract obtained by analysis is dispersed in water to obtain 1.1g crude drug / ml sample solution, which is dynamically adsorbed by 800g AB-8 macroporous adsorption resin. After the adsorption is completed, wash with water first When the color is light, it is eluted with 60% ethanol. The eluate is concentrated under reduced pressure. The concentrated solution is adsorbed on 250g polyamide resin. After the adsorption is completed, it is washed with water and then eluted with 70% ethanol. Collect the ethanol eluate. It was concentrated under reduced pressure, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for extracting flavonoids from plants, particularly a method for extracting the flavonoids from phyllostachys pubescens leaves. The method comprises the following steps: crushing dried phyllostachys pubescens leaves; extracting by using a supercritical carbon dioxide fluid extraction method, wherein ethanol is taken as a carrier; adsorbing the obtained extract by using a macroporous resin column; washing the resin column until the color of the effluent becomes light; eluting with ethanol solution; performing vacuum concentration on the eluent until no alcohol exists; adding polyamide resin column for adsorbing; properly washing the resin column; eluting with ethanol solution; performing vacuum concentration on the eluent; and performing spray drying on the concentrated solution to obtain the phyllostachys pubescens leaf total flavone product. The preparation method is easy to operate, and is an environment-friendly preparation method, and the content of the total flavone in the obtained phyllostachys pubescens leaf extract is over 50 percent.

Description

technical field [0001] The invention belongs to the field of separation and purification of natural products, and relates to a preparation process of effective substances in natural products, in particular to a method for extracting flavonoids from moso bamboo leaves. Background technique [0002] Bamboo belongs to Monocotyledonous Plants, Poaceae ( grainier ) Bamboo subfamily (bambusoideae ) Perennial evergreen plants are one of the most practical plants in the world today. There are more than 1,300 species of bamboo in 70-80 genera in the world, and there are more than 500 species of bamboo in 39 genera in my country, of which Moso bamboo ( phyllostachys pubescens ) is very rich in resources and widely distributed in the southern provinces of my country. Moso bamboo is widely used, and bamboo shoots, stems, bark, roots, etc. have all been developed and utilized, but moso bamboo leaves have not been fully utilized and developed, and the advantages and potential of bamb...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/899
Inventor 王晖陈梅荣赖娟华刘良玉庄妍朱红
Owner 江西省药物研究所
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com