Culter alburnus gynogenesis inducing method

A technology for gynogenesis and gynogenesis of the cyper, which is applied in the field of induction of gynogenesis of the cyper, can solve problems such as no reports on gynogenesis induction of the cyper, and achieve easy identification, simplified identification procedures, and strong operability Effect

Active Publication Date: 2014-02-12
ZHEJIANG INST OF FRESH WATER FISHERIES
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  • Culter alburnus gynogenesis inducing method
  • Culter alburnus gynogenesis inducing method
  • Culter alburnus gynogenesis inducing method

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Embodiment Construction

[0034] The method for inducing the gynogenesis of the crocodile chinensis of the present invention is specifically as follows:

[0035] It involves joint links such as enhanced parental cultivation, artificial oxytocin, inactivation of sperm genetic material, and doubling of fertilized egg chromosomes. The specific technical process and parameters are as follows:

[0036] 1. Strengthen cultivation. The primary condition for the success of this technical invention is to obtain the parents of the gonads of the Culper and carp with good gonad development. From December to January of the next year, select from the lakes or ponds more than 3 years old, no disease and no injury, and a body weight of more than 1.0kg. The improved species of Clamella is cultivated in a special pond, and the ratio of male to female is 2:1. During the cultivation period, high-quality extruded compound feed is fed at the beginning, and some fresh and live bait is added later, such as fresh and live sma...

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Abstract

The invention discloses a culter alburnus gynogenesis inducing method. The culter alburnus gynogenesis inducing method comprises the following steps that (1) cyprinoid seminal fluid is collected; (2) ultraviolet inheritance inactivation processing is carried out on the cyprinoid seminal fluid; (3) mature eggs of culter alburnus are collected, and dry insemination is carried out on the cyprinoid seminal fluid after ultraviolet inheritance inactivation processing; (4) cold shock processing is carried out on fertilized eggs, then the fertilized eggs are put into an incubation container to be incubated, and normally alive fries become gynogenesis fish. According to the culter alburnus gynogenesis inducing method, ultraviolet inheritance inactivation sperms of common cyprinoid are adopted to stimulate the culter alburnus eggs, release of second polar bodies of the eggs is restrained through cold shock to promote chromosome doubling, and culter alburnus individuals are successfully obtained. Normally alive individuals are the gynogenesis fish, subsequent identification analyses of chromosomes, genetic markers and the like are not needed, and identification procedures are significantly simplified.

Description

technical field [0001] The invention relates to fish biotechnology, in particular to a method for inducing the gynogenesis of the cichlid cichlid. Background technique [0002] Crawfish ( Culter alburnus Basilewsky) is one of the important freshwater economic fishes in my country. At present, most of the aquaculture Culturus still remain in the use of natural broodstock or multi-generation selected cultured species. Not only have their breeding traits not been explored, but also due to improper parent selection, inbreeding, etc., the breeding traits have appeared slow growth, Different degrees of germplasm decline such as meat quality decline. Artificial gynogenesis induction is an important technical means to quickly fix the traits of the female parent and sex control in fish, which can speed up the breeding process and efficiency of fish, especially fish with a long generation cycle. Studies have shown that one artificial gynogenesis induction is equivalent to 14 gener...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 顾志敏贾永义
Owner ZHEJIANG INST OF FRESH WATER FISHERIES
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