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A kind of purification method of pentose compound

A technology of pure product and sodium chloride aqueous solution is applied in the field of preparing pure product of fondaparinux sodium, which can solve problems such as difficult operation and complicated operation method, achieve short operation process, high yield and purity, and reduce difficulty Effect

Active Publication Date: 2017-08-25
BRIGHTGENE PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In this method, the activated carbon needs to be fixed on the two-dimensional cellulose disc with a resin auxiliary agent, and the activated carbon needs to be circulated for 2 hours, and then needs to be filtered through a microporous membrane. The actual operation method is complicated and difficult to operate.

Method used

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  • A kind of purification method of pentose compound
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Effect test

Embodiment 1

[0040] Uni Q-50S (particle size 67 μm, pore size 500 angstroms, purchased from Nanomicro Technology) was loaded into a medium-pressure preparative chromatographic column (46×540 mm). Dissolve 10 g of fondaparinux sodium (purity about 70%) in 1 L of water and load the sample. After loading the sample, wash one column volume with water, and use 0.2-1.0M NaCl aqueous solution gradient elution at a flow rate of 30ml / min. The fondaparinux sodium was collected, combined, desalted and concentrated to obtain 6.0 g of fondaparinux sodium with a purity of 99.7%.

Embodiment 2

[0042] Uni Q-50S (particle size 67 μm, pore size 500 angstroms, purchased from Nanomicro Technology) was loaded into a medium-pressure preparative chromatographic column (49×310 mm). Dissolve 10 g of fondaparinux sodium (purity about 70%) in 1 L of water and load the sample. After loading the sample, wash one column volume with water, and use 0.2-1.0M NaCl aqueous solution gradient elution at a flow rate of 30ml / min. The fondaparinux sodium was collected, combined, desalted and concentrated to obtain 5.3 g of fondaparinux sodium with a purity of 99.6%.

Embodiment 3

[0044] Uni Q-50S (particle size 67 μm, pore size 500 angstroms, purchased from Nanomicro Technology) was loaded into a medium-pressure preparative chromatographic column (15×310 mm). Dissolve 10 g of fondaparinux sodium (purity about 70%) in 1 L of water and load the sample. After loading the sample, wash one column volume with water, and use 0.2-1.0M NaCl aqueous solution gradient elution at a flow rate of 20ml / min. The fondaparinux sodium was collected, combined, desalted and concentrated to obtain 5.7 g of fondaparinux sodium with a purity of 99.6%.

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Abstract

The invention relates to a method for purifying pentose compounds with an anion-exchange chromatographic column. The method comprises loading crude pentoses on an anion-exchange chromatographic column and eluting with an aqueous solution. The method has high purification efficiency and is easy to operate.

Description

technical field [0001] The present invention relates to a kind of purification method of pentose, more specifically relate to a kind of method for preparing the pure product of fondaparinux sodium. Background technique [0002] Fondaparinux Sodium (Fondaparinux Sodium) is a completely synthetic sodium salt of pentasaccharide sulfate, which has a chemical structure as shown in the following formula (I): [0003] [0004] (I). [0005] Fondaparinux is an indirect inhibitor of antithrombin-dependent factor Xa. It has been marketed in France, the United Kingdom, Germany, China and the United States. It is clinically used for the treatment and prevention of deep venous thromboembolic events, and has a good antithrombotic effect. [0006] Due to the complicated structure of fondaparinux sodium and long synthetic route, various impurities will be formed in the reaction process, which will affect the safety of the product. And the existence of impurity itself can affect the st...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/04C07H1/06
Inventor 袁建栋蒋翠英
Owner BRIGHTGENE PHARMA