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Quick breeding method for novel triploid salvia miltiorrhiza detoxification varieties

A triploid, new variety technology, applied in horticultural methods, botanical equipment and methods, plant genetic improvement, etc., can solve problems such as breakthroughs in cross-breeding of Salvia miltiorrhiza that have not yet been seen, and achieve the problem of reducing the yield of medicinal materials of Salvia miltiorrhiza. Yield and pharmaceutical active ingredients increase, the effect of vigorous growth

Active Publication Date: 2014-02-19
陈瑞阳
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Problems solved by technology

However, there has been no breakthrough in cross breeding of Salvia miltiorrhiza

Method used

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  • Quick breeding method for novel triploid salvia miltiorrhiza detoxification varieties
  • Quick breeding method for novel triploid salvia miltiorrhiza detoxification varieties
  • Quick breeding method for novel triploid salvia miltiorrhiza detoxification varieties

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Embodiment 1

[0035]In October 2012, two mutated triploid Salvia miltiorrhiza plants were found in the triploid Salvia miltiorrhiza production field. We tentatively named them: RY1, RY2. Subsequently, we collected the new young leaves of these two mutant plants, and carried out detoxification treatment and tissue culture rapid propagation: first, wash the young leaves with running water, wash away the mud and sand, and in the ultra-clean workbench, according to the aseptic operation procedure, use 75% alcohol disinfection for 30-60 seconds → 0.1% mercury liter disinfection for 15 minutes, rinse with sterile water 5 times, cut the material into 1-2 square centimeters with a scalpel, inoculate in 1 / 2MS + 6BA0.5-1mg / L culture Basically, cluster buds are directly induced, and 20-30 days after inoculation, after a large number of cluster buds are produced, a large number of cloning and proliferation of test tube seedlings are carried out, and when the cluster buds grow true leaves, transfer to 1...

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Abstract

The invention relates to a quick breeding method for novel triploid salvia miltiorrhiza detoxification varieties. The method comprises the following steps: detoxifying steam tips or newly born leaves of triploid salvia miltiorrhiza, taking the detoxified material as a tissue culture material, and inoculating the detoxified material on a 1 / 2 MS culture medium, and cultivating at 20-25 DEG C with 3000 Lux of light strength under the light period of 14 h / d till multiple shoots are differentiated from cut surfaces of leaf margins; carrying out bottle expand reproduction; reproducing 3-5 seedlings in each bottle; carrying out bottle seedling transplantation when new roots are 3-5 centimeters; carrying out field cultivation management according to the conventional triploid salvia miltiorrhiza field cultivation method; measuring the contents of tanshinone II A, danshinolic acid B and cryptotanshinone; confirming the breeding result of the novel triploid salvia miltiorrhiza detoxification varieties according to the measuring results of the medicine yields and the contents of effective components of main medicines of the novel triploid salvia miltiorrhiza detoxification varieties. The novel triploid salvia miltiorrhiza detoxification varieties can be bred in two years, so that the degradation problem of the novel salvia miltiorrhiza varieties caused by biotic and abiotic stress is solved; theoretically, the advantages of the detoxified triploid salvia miltiorrhiza can be kept for hundred years.

Description

technical field [0001] The invention relates to a breeding method for new plant varieties, in particular to a rapid breeding method for new triploid salvia miltiorrhiza miltiorrhiza (lines) free of viruses. Background technique [0002] Salvia ( Salvia miltiorrhiza Bunge) is one of the earliest and most widely used traditional Chinese medicines in my country. It is a famous medicine for promoting blood circulation and removing stasis. With the aging population in our country, cardiovascular disease has become a major disease that endangers human health, the incidence rate is increasing year by year, and the demand for drugs is increasing. At present, there are more than 100 kinds of compound Chinese medicines produced with Danshen as raw materials in my country, and there is a huge demand for high-quality Danshen medicinal materials. Since the 1960s and 1970s, after the successful conversion of wild salvia miltiorrhiza to domestic species, production has developed greatly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H1/04
Inventor 陈瑞阳
Owner 陈瑞阳
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