Human anti-HBV surface antigen genetic engineering antibody, and preparation method and application thereof

A technology of genetically engineered antibodies and surface antigens, applied in genetic engineering, antiviral agents, antiviral immunoglobulins, etc., can solve problems such as mass production limitations, limited applications, and infections
CN103588874AActive Publication Date: 2014-02-19中国疾病预防控制中心病毒病预防控制所
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
中国疾病预防控制中心病毒病预防控制所
Publication Date
2014-02-19

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Abstract

The invention discloses a human anti-HBV surface antigen genetic engineering antibody, and a preparation method and application thereof. According to the invention, the phage surface display technology is adopted, peripheral blood lymphocyte of person having high titer surface-antibody after immunization of hepatitis B vaccine is collected is obtained, a human anti-HBV surface antigen genetic engineering antibody library is established through a genetic engineering means, and a Fab section of the specific anti-HBV surface antigen genetic engineering antibody is obtained through screening. The obtained Fab section of the antibody is named to be HBFab21. The amino acid sequence of a light chain variable region of the HBFab21 is shown in SEQ ID No.1, and the amino acid sequence of a heavy chain variable region of the HBFab21 is shown in SEQ ID No.2. According to the invention, a foundation for hepatitis B virus infection prevention and researches on treatment to hepatitis B virus is laid; through the foundation, an antibody product having an effect of neutralizing hepatitis B virus infection is obtained, and can be applied to preparation of a clinical drug or diagnostic reagent for liver diseases relating to hepatitis B virus.
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Description

technical field

[0001] The invention relates to genetic engineering antibody technology, in particular to a human-sourced anti-hepatitis B virus surface antigen genetic engineering antibody; the invention also relates to the application of the antibody in the preparation of drugs for preventing or treating hepatitis B virus-related liver diseases. Background technique

[0002] Hepatitis B virus infection is a major public health problem worldwide. After acute hepatitis B, 5%-10% of persistent HBV infection develops into chronic liver diseases including chronic active hepatitis, liver cirrhosis and primary hepatocellular carcinoma. It is estimated that 500,000 to 1,000,000 people worldwide die each year from various liver diseases related to HBV. my country is a big country with hepatitis, and the carrier rate of hepatitis B virus is 9.75% of the population. There are 420 million hepatitis B virus infected people in the world, and about 130 million people in my country are c...

Claims

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