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Determination method for tanshinol content in Naozhengning preparation

A measurement method, the technology of brain shock, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of no quantitative determination of active ingredient content, etc., and achieve the effects of short measurement time, improved quality and good repeatability

Active Publication Date: 2015-02-11
SHANXI ZHENDONG ANXIN BIOLOGICAL PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the standards including Naozhenning Granules and Wutang Granules only have qualitative identification tests, and do not involve any quantitative determination of the content of active ingredients

Method used

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  • Determination method for tanshinol content in Naozhengning preparation
  • Determination method for tanshinol content in Naozhengning preparation
  • Determination method for tanshinol content in Naozhengning preparation

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Naozhenning Granules (according to the Ministry of Health drug standard WS 3 - Determination of Danshensu content in granules made from -B-3312-98).

[0030] Prescription: Angelica 200g, Rehmannia glutinosa 200g, Moutan bark 150g, Chuanxiong 150g, Dilong 150g, Salvia miltiorrhiza 375g, Poria cocos 250g, Chenpi 250g, Zhuru 250g, Suanzaoren (fried) 250g, Baiziren 250g.

[0031] Preparation method: the above eleven flavors, angelica, peony cortex, Chuanxiong, and tangerine peel are distilled to extract volatile oil, and the distilled aqueous solution is collected in a separate device; the dregs and the remaining seven flavors such as salvia miltiorrhiza are decocted twice, each time for 1.5 hours, and the decoction is combined. After filtration, the filtrate was combined with the above aqueous solution, concentrated to a clear paste with a relative density of 1.35-1.38 (55°C), added appropriate amount of sucrose and dextrin, granulated, dried, added the above-mentioned vol...

Embodiment 2

[0040]Determination of Danshensu content in Naozhenning sugar-free granules (sugar-free granules made according to the Ministry of Health drug standard YBZ27112005).

[0041] Prescription: Angelica 333.4g, Rehmannia glutinosa 333.4g, Moutan bark 250g, Chuanxiong 250g, Dilong 250g, Salvia miltiorrhiza 625g, Poria 416.7g, Chenpi 416.7g, Zhuru 416.7g, Suanzaoren (fried) 416.7g, Baiziren 416.7g.

[0042] Preparation method: the above eleven flavors, angelica, peony cortex, Chuanxiong, and tangerine peel are distilled to extract volatile oil, and the distilled aqueous solution is collected in a separate device; the dregs and the remaining seven flavors such as salvia miltiorrhiza are decocted twice, each time for 1.5 hours, and the decoction is combined. Filter, combine the filtrate with the above aqueous solution, concentrate to a clear paste with a relative density of 1.35-1.38 (55°C), use dextrin as an auxiliary material, granulate, dry, add the above-mentioned volatile oil, mix ...

Embodiment 3

[0051] Naozhenning Tablets (Based on ZL93100741 and Ministry of Health Drug Standard WS 3 - Determination of Danshensu content in tablets made from -B-3312-98).

[0052] Prescription: Angelica 1000g, Rehmannia glutinosa 1000g, Moutan bark 750g, Chuanxiong 750g, Dilong 750g, Salvia miltiorrhiza 1875g, Poria cocos 1250g, Chenpi 1250g, Zhuru 1250g, Suanzaoren (fried) 1250g, Baiziren 1250g.

[0053] Preparation method: the above eleven flavors, angelica, peony cortex, Chuanxiong, and tangerine peel are distilled to extract volatile oil, and the distilled aqueous solution is collected in a separate device; the dregs and the remaining seven flavors such as salvia miltiorrhiza are decocted twice, each time for 1.5 hours, and the decoction is combined. After filtering, the filtrate was combined with the above aqueous solution, concentrated to an appropriate amount of clear paste, added ethanol to make the alcohol content 60%, mixed evenly, allowed to stand for precipitation, filtered,...

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Abstract

The invention provides a determination method for tanshinol content in a Naozhengning preparation. Salvianic acid A sodium in the preparation is extracted by using a methanol-glacial acetic acid or methanol-trifluoroacetic acid solution to prepare a solution for test products, octadecylsilane chemically bonded silica chromatographic column is adopted, the methanol to 0.01-0.2 percent of trifluoroacetic acid solution, which equals to (5:95) to (20:80), is used as a mobile phase for carrying out high performance liquid chromatographic analysis, the chromatographic peak of the salvianic acid A sodium is determined the in the position of 280 nm wave length, and the tanshinol content is calculated through an external standard method. The determination method for the tanshinol content, established in the invention, has strong specificity, the linear relation is good, the accuracy is high, the repeatability is good, the determining time is short, the tanshinol content is used as a quality control index in the Naozhengning preparation, the product quality of the Naozhengning preparation can be effectively controlled, and the transfer rate of the salvianic acid A sodium can be appraised more scientifically.

Description

technical field [0001] The invention relates to a method for determining the content of danshensu, in particular to a method for determining the content of danshensu in Naozhenning preparation. Background technique [0002] The Naozhenning preparation is prepared according to the prescription disclosed in ZL93100741 in various suitable dosage forms such as granules, capsules, tablets, soft capsules, and oral liquids, and is used for the prevention and treatment of traumatic brain injury syndrome. [0003] Naozhenning Granules are included in the "Drug Standards of the Ministry of Health of the People's Republic of China Traditional Chinese Medicine Prescriptions (Volume 17)" on page 230 (standard number: WS 3 -B-3312-98), the prescription is: Angelica 200g, Dihuang 200g, Moutan bark 150g, Chuanxiong 150g, Dilong 150g, Salvia miltiorrhiza 375g, Poria cocos 250g, Chenpi 250g, Zhuru 250g, Suanzaoren (fried) 250g, Baizi Jen 250g; the preparation method is: the above eleven flav...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 姚娟娟吴月侠朱平李安平秦正国南莹
Owner SHANXI ZHENDONG ANXIN BIOLOGICAL PHARM CO LTD