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Method for measuring naringin content in preparation for reunion of bone

A technology for connecting bones and tendons and measuring methods, which can be used in anti-inflammatory agents, bone diseases, measuring devices, etc., can solve the problems of lack of content measuring methods, etc., and achieve the effects of strong specificity, improved quality, and good repeatability.

Active Publication Date: 2011-03-16
山西华元医药生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Jiegu Xujin Tablets are recorded on page 170 of volume 11 of the Ministry of Health's drug standard "Prescriptions of Traditional Chinese Medicine", standard number WS 3 -B-2226-96, the prescription is Lizard 500g, Drynaria 333g, Panshanlong 167g, the dosage form is sugar-coated tablet, function of promoting blood circulation and removing blood stasis, reducing swelling and relieving pain, mainly treating soft tissue injury, fracture, etc., but lacks content determination method

Method used

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  • Method for measuring naringin content in preparation for reunion of bone
  • Method for measuring naringin content in preparation for reunion of bone
  • Method for measuring naringin content in preparation for reunion of bone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Jiegu Xujin Tablets (according to the Ministry of Health drug standard WS 3 -Determination of naringin content in the sugar-coated tablet made by B-2226-96 prescription).

[0042] 1) Chromatographic conditions and system suitability test:

[0043] Chromatographic column: octadecylsilane bonded silica gel column (5μm, 4.6mm×200mm);

[0044] Mobile phase: methanol: 2.25% acetic acid solution = 35: 65;

[0045] Flow rate: 0.9mL·min -1 ;

[0046] Detection wavelength: 283nm;

[0047] Column temperature: 35°C.

[0048] The number of theoretical plates is calculated according to the peak of naringin, and should not be less than 2000.

[0049] 2) Preparation of reference substance solution: Accurately weigh 10mg of naringin reference substance dried at 110°C to constant weight, put it in a 100mL measuring bottle, add methanol to the mark, shake well, accurately measure 3mL, put it in a 10mL measuring bottle , add methanol 2mL, and then dilute to the mark with 2.25% acet...

Embodiment 2

[0055] Jiegu Xujin Capsules (according to the Ministry of Health drug standard WS 3 -Determination of naringin content in capsules made by B-2226-96 prescription, specification: each capsule contains 0.4g).

[0056] 1) Chromatographic conditions and system suitability test:

[0057] Chromatographic column: octadecylsilane bonded silica gel column (10μm, 4.6mm×250mm);

[0058] Mobile phase: methanol: 2.25% acetic acid solution = 38: 62;

[0059] Flow rate: 1.0mL·min -1 ;

[0060] Detection wavelength: 283nm;

[0061] Column temperature: 40°C.

[0062]The number of theoretical plates is calculated according to the peak of naringin, and should not be less than 2000.

[0063] 2) Preparation of reference substance solution: Accurately weigh 3mg of naringin reference substance, put it in a 10mL measuring bottle, add methanol to the mark, shake well, accurately measure 1mL, put it in a 10mL measuring bottle, add 4mL of methanol, and then use 2.25 Dilute the % acetic acid solut...

Embodiment 3

[0069] Jiegu Xujin Capsules (according to the Ministry of Health drug standard WS 3 -Determination of naringin content in capsules made by B-2226-96 prescription, specification: each capsule contains 0.4g).

[0070] 1) Chromatographic conditions and system suitability test:

[0071] Chromatographic column: octadecylsilane bonded silica gel column (10μm, 4.6mm×250mm);

[0072] Mobile phase: methanol: 2.25% acetic acid solution = 40:60;

[0073] Flow rate: 1.2mL·min -1 ;

[0074] Detection wavelength: 283nm;

[0075] Column temperature: 45°C.

[0076] The number of theoretical plates is calculated according to the peak of naringin, and should not be less than 2000.

[0077] 2) Preparation of reference substance solution: Accurately weigh 15mg of naringin reference substance dried at 110°C to constant weight, put it in a 50mL measuring bottle, add methanol to the mark, shake well, accurately measure 1mL, put it in a 10mL measuring bottle , add methanol 4mL, and then dilute...

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Abstract

The invention relates to a method for measuring the content of naringoside in a muscle and bone reunion preparation. The method selects the naringoside as an index composition of rhizoma drynariae in the muscle and bone reunion preparation, uses methanol and an acetic acid solution to prepare a reference substance solution of the naringoside and a test sample solution of the muscle and bone reunion preparation, adopts octadecyl silane bonded silica gel column to carry out high-efficiency liquid phase chromatography mensuration under the 283 nm detection wavelength by taking the ratio of the methanol to 1.5 to 2.5 percent the acetic acid solution as 35:65 to 40:60 as a mobile phase, and uses an external standard method to calculate the content of the naringoside in a muscle and bone reunion preparation sample. The established mensuration method has strong specificity, good linear relation, high accuracy, good repeatability and short measuring time, can scientifically evaluate the transfer rate of the naringoside according to the method, and effectively improve the quality of a product.

Description

technical field [0001] The invention belongs to the technical field of content determination of Chinese patent medicines, and relates to a content determination method of Chinese patent medicine containing drynariae, in particular to the content determination of drynariae in the preparation of rhizoma rhizomes. Specifically, the present invention uses naringin in drynariae as an index component to measure the content. Background technique [0002] Jiegu Xujin Tablets are recorded on page 170 of volume 11 of the Ministry of Health's drug standard "Prescriptions of Traditional Chinese Medicine", standard number WS 3 -B-2226-96, the prescription is Lizard 500g, Rhizoma Drynaria 333g, and Panshanlong 167g. The dosage form is sugar-coated tablet. [0003] Rhizoma Drynariae is the main flavor of the bone-replenishing tendon tablets (sugar-coated tablets) and the film-coated tablets, dispersible tablets, capsules, granules and other preparations made according to the prescription ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/8945A61P19/08A61P29/00G01N30/36A61K35/56A61K35/583
Inventor 朱平李娜王志敏
Owner 山西华元医药生物技术有限公司